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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1978-05-01 to 1978-08-01
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Followed guideline, well documented, with analytical.
Cross-reference
Reason / purpose:
reference to same study

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1979
Report Date:
1979
Reference Type:
publication
Title:
Unnamed
Year:
1981

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: US EPA, 1975. Reliability, deviations and validity evaluated against the criteria of USEPA 850.1075, 1996.
Deviations:
no
Principles of method if other than guideline:
Not applicable
GLP compliance:
no
Remarks:
study pre-dates GLPs

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Linear alkylbenzene sulfonate, C11.8 LAS (Anionic surfactant) (X0029.01)
- Structure provided in the study report
- Substance type: Pure active substance
- Physical state: Not available
- Stability under test conditions: Not available
- Storage condition of test material: Not available
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
Not applicable

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations: Water samples for determination of test concentrations were collected at the beginning and end of each test.
- Sampling method: Samples were preserved with 1% formalin.
- Sample storage conditions before analysis: Not available

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Stock solutions were prepared by diluting the materials in deionized water to the desired concentrations.
- Eluate: Not applicable
- Differential loading: Not applicable
- Controls: Prepared using deionized water.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Not applicable
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): Not applicable
- Evidence of undissolved material (e.g. precipitate, surface film, etc): None

Test organisms

Test organisms (species):
Lepomis macrochirus
Details on test organisms:
TEST ORGANISM
- Common name: Bluegill
- Strain: Not available
- Source: Not available
- Age at study initiation (mean and range, SD): Juvenile
- Length at study initiation (length definition, mean, range and SD): Mean standard length: 4.2 cm, range: 3.0-5.1 cm
- Weight at study initiation (mean and range, SD): Average: 1.1 g, range: 0.5-1.9 g
- Method of breeding: Not available
- Feeding during test: Not fed
- Food type: Not applicable
- Amount: Not applicable
- Frequency: Not applicable


ACCLIMATION
- Acclimation period: At least 10 days
- Acclimation conditions (same as test or not): Same as test
- Type and amount of food: Not available
- Feeding frequency: Not available
- Health during acclimation (any mortality observed): Not available


QUARANTINE (wild caught)
- Duration: Not applicable
- Health/mortality: Not applicable

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
Not applicable

Test conditions

Hardness:
Total hardness was 137 mg/L as CaCO3.
Test temperature:
Temperature was maintained at 20 +/- 2 degree C.
pH:
The pH varied from 7.8 to 8.1
Dissolved oxygen:
Dissolved oxygen in the fish tests averaged 9.0 (ranged 8.4-9.6) mg/L.
Salinity:
Not applicable
Nominal and measured concentrations:
Details of nominal and measured test concentrations are not available in the study report. Measured concentrations were 79 % of nominal concentrations. Results are based on measured concentrations.
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open / closed: Not available
- Material, size, headspace, fill volume: 15 liter glass aquaria containing 10 liters of dilution water.
- Aeration: Not available
- Type of flow-through (e.g. peristaltic or proportional diluter): Not applicable
- Renewal rate of test solution (frequency/flow rate): Not applicable
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 1.1 g/L


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The dilution water was a blend of well water and deionized water, mixed to the desired hardness.
- Total organic carbon: Not available
- Particulate matter: Not available
- Metals: 10 heavy metals were measured, all were <0.2 mg/L. See report.
- Pesticides: Not available
- Chlorine: Not available
- Alkalinity: Not available
- Ca/mg ratio: Not available
- Conductivity: Not available
- Culture medium different from test medium: Not available
- Intervals of water quality measurement: Dissolved oxygen, pH and hardness were monitored twice during the test.


OTHER TEST CONDITIONS
- Adjustment of pH: Not applicable
- Photoperiod: 16 hours illumination
- Light intensity: Not available


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Fish mortality recorded 1, 3 and 5 hours after test initiation, and daily until test termination.


TEST CONCENTRATIONS
- Spacing factor for test concentrations: Not available
- Justification for using less concentrations than requested by guideline: Not applicable
- Range finding study: No
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
1.67 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
act. ingr.
Basis for effect:
mortality
Remarks on result:
other: 95% CL: 1.58-1.77mg/L
Details on results:
- Behavioural abnormalities: None
- Observations on body length and weight: None
- Other biological observations: None
- Mortality of control: None
- Other adverse effects control: None
- Abnormal responses: None
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None
- Effect concentrations exceeding solubility of substance in test medium: None
Results with reference substance (positive control):
Not applicable
Reported statistics and error estimates:
Probit analysis of all mortality data in this study followed the procedures of Finney (1971).

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
All the criteria of USEPA OPPTS 850.1075, 1996 were met
Conclusions:
The 96 h LC50 of C11.8 LAS (linear alkylbenzene sulfonate) to bluegill (Lepomis macrochirus) was 1.67 mg/L, based on mean measured concentrations of active ingredient.
Executive summary:

In a 96 h acute toxicity study, Bluegill (Lepomis macrochirus) were exposed to C11.8 LAS (linear alkylbenzene sulfonate, an anionic surfactant) under static conditions. Nominal concentrations were not specified in the report. Measured concentrations were 79 % of nominal concentrations.

The 96 h LC50 value, based on measured concentration, was 1.67 mg a.i./L, with 95% CL 1.58 to 1.77 mg/L.

This toxicity study is classified as acceptable and satisfies the guideline requirement for the current USEPA OPPTS 850.1075 (1996) acute fish toxicity study.