Benzenesulfonic acid, 4-C10-13-sec-alkyl derivs.

Long-term toxicity to aquatic invertebrates

Some information in this page has been claimed confidential.

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1976-04 to 1977-02

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well documented, equivalent to guideline

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1977

Report date:

1977

Reference 2

Reference Type:

publication

Title:

Unnamed

Year:

1979

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

Deviations:

no

GLP compliance:

no

Remarks:

Study pre-dates GLP

Test material

Test material information

Constituent 1

Reference substance name:

Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts

EC Number:

270-115-0

EC Name:

Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts

Cas Number:

68411-30-3

Molecular formula:

Not applicable for UVCB

IUPAC Name:

sodium 4-undecylbenzenesulfonate

Constituent 2

Details on test material:

- Name of test material: C11.8 LAS; Linear Alkylbenzene Sulfonate; (TSIN = APS-71.031)
- Substance type: Pure active substance
- Physical state: N/A

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: all concentrations
- Sampling method: Samples were taken from test vessels on day 0, and every 3-5 days through out the 21-day test.
- Sample storage conditions before analysis: Preserved with 1% formaldehyde and stored at 5 C until analysis.

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Direct dilution of the appropriate amount of test substance into blended well water
- Controls: Blended well water
- Evidence of undissolved material: no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain/clone: N/A
- Source: In-house culture
- Age of parental stock: not specified
- Age of test organisms: <12 h old at beginning of test
- Feeding during test: yes
- Food type: ground trout chow/alfalfa mixture, 1000 mg/L
- Amount: 5 mg/L
- Frequency: Each diluter cycle

ACCLIMATION
- Acclimation period: Not specified
- Acclimation conditions (same as test or not): N/A
- Type and amount of food: N/A
- Feeding frequency: N/A
- Health during acclimation (any mortality observed): N/A

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: Young were manually siphoned from existing culture units.

Study design

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Test conditions

Hardness:

120 +/- 18 mg/L CaCO3

Test temperature:

21 +/- 1 C

pH:

7.5 +/- 0.2 SU

Dissolved oxygen:

8.9 +/- 0.4 mg/L

Salinity:

N/A

Nominal and measured concentrations:

The nominal test concentrations were not specified in the study report. Mean Measured concentrations were 0, 0.32, 0.59, 1.18, 2.52, and 4.85 mg/L C11.8 LAS (active ingredient).

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: open
- Material: 1 liter glass beakers, 1 L test solution
- Aeration: N/A (test was flow-thru)
- Type of flow-through: Mount and Brungs proportional diluter, with modifications
- Renewal rate of test solution: 125 ml per 20 min cycle, or one chamber volume every 2.5 hr.
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 5 daphnids/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Blended well water (a mixture of well water and deionized water)
- Total organic carbon: N/A
- Particulate matter: N/A
- Metals: N/A
- Pesticides: N/A
- Chlorine: N/A
- Alkalinity: N/A
- Ca/mg ratio: N/A
- Conductivity: N/A
- Salinity: N/A
- Culture medium different from test medium: No
- Intervals of water quality measurement: Daily

OTHER TEST CONDITIONS
- Adjustment of pH: N/A
- Photoperiod: N/A
- Light intensity: N/A

EFFECT PARAMETERS MEASURED: Mortality, 24 hr, 96 hr, 7 days, then daily to end of test. Reproduction, daily beginning day 7 or 8 to end of test.

RANGE-FINDING STUDY: no

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

1.18 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

act. ingr.

Basis for effect:

other: Parent (F0) Survival and reproduction (Total # young in 21 days; and Mean Brood Size)

Effect concentrations 2

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

1.5 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

act. ingr.

Basis for effect:

other: Total young production

Remarks on result:

other: 95% CL 0.75-3.33 mg/L

Effect concentrations 3

Duration:

21 d

Dose descriptor:

LC50

Effect conc.:

1.67 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

act. ingr.

Basis for effect:

mortality

Remarks on result:

other: 95% CL 1.28-2.18 mg/L

Effect concentrations 4

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

2.3 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

act. ingr.

Basis for effect:

other: Average brood size

Remarks on result:

other: 95% CL 1.62-3.55 mg/L

Effect concentrations 5

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

2.31 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

act. ingr.

Basis for effect:

other: Percentage of days reproduction occurred

Remarks on result:

other: 95% CL 0.30-4.18 mg/L

Effect concentrations 6

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

3.94 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

act. ingr.

Basis for effect:

mortality

Remarks on result:

other: 95% CL 2.87-6.83 mg/L

Details on results:

- Mortality of parent animals: Controls, 10%
- No. of offspring produced per Control: 39
- Body length and weight of parent animals: N/A
- Type and number of morphological abnormalities: N/A
- Type and number of behavioural abnormalities: N/A
- Number of males and females (parental): N/A
- Time to first brood release or time to hatch- 7 or 8 days
- Egg development time: N/A
- Brood size: Control, 8.0 (mean)
- Time to sexual maturity: N/A
- Type and magnitude of biochemical changes: N/A
- Other biological observations: N/A
- Effect concentrations exceeding solubility of substance in test medium: no

Reported statistics and error estimates:

All results were calculated with a computerized probit analysis program after Finney (1964, 1971). Survival data for 96 hr and 21 days was used to calculate LC50 values and confidence limits. All results were calculated based on mean measured concentrations of test material.

Any other information on results incl. tables

Chronic Toxicity of C11.8 LAS to Daphnia magna (study # 20710)

Mean Measured Test Conc (mg/L)	% Survival	Total Young Produced	Mean Brood Size	% of Days that Reproduction Occurred
0	90	786	8.0	82
0.32	85	1445	11.7	96
0.59	85	1131	9.7	90
1.18	75	1036	10.7	86
2.52	40	477	7.9	71
4.85	0	0	0	0

N=20 organisms at beginning of test (4 replicates of 5 organisms each).

LAS = linear alkylbenzene sulfonate.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 21 day NOEC of C11.8 LAS to Daphnia magna was 1.18 mg/L, based on survival and reproduction. The 21 day LC50 was 1.67 mg/L. Results are based on the mean measured concentration of the active ingredient.

Executive summary:

The long-term toxicity of C11.8 LAS (linear alkylbenzene sulfonate)) was evaluated in a 21 day survival and reproduction test with Daphnia magna, following the OECD 211 guideline. Four replicates per concentration, 5 organisms per replicate, were tested in a flow-through system (a modified Mount and Brungs proportional diluter). Mean measured concentrations were: Control, 0.32, 0.59, 1.18, 2.52, and 4.85 mg/L active ingredient (C11.8 LAS).

 

Survival was monitored at 24 hr, 96 hr, 7 days and daily thereafter. Reproduction was monitored beginning with the production of the first brood on day 7 or 8, and daily thereafter. LC50s and EC50s were calculated by probit analysis. Results are based on the mean measured concentration of the active ingredient.

 

The 21-day NOEC was 1.18 mg/L, based on both survival and reproduction. The 21-day LC50 was 1.67 mg/L (95% CL 1.28 -2.18 mg/L). The 21-day EC50s, based on total young production, average brood size, and % days reproduction occurred, were 1.50, 2.30, and 2.31 mg/L, respectively.