heptan-2-yl [(5-chloroquinolin-8-yl)oxy]acetate

Toxicity to terrestrial arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to bees: acute oral

Remarks:

Acute toxicity via contact was also assessed.

Type of information:

experimental study

Adequacy of study:

key study

Study period:

24 August 1987 - 30 August 1987

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

equivalent or similar to guideline

Guideline:

other: Working Document D3 of the United Kingdom Pesticide Safety Precautions Scheme

Deviations:

not specified

Principles of method if other than guideline:

Test done in accordance with the regulations: EPA FIFRA 71-2

GLP compliance:

yes

Application method:

other: Both oral and contact

Analytical monitoring:

no

Details on sampling:

Not applicable

Vehicle:

yes

Details on preparation and application of test substrate:

Contact
- Method of test material application: single topical dose
- Body part: thorax
- Volume of test solution applied: 1.0 µL single dose of the appropriate concentration
- Controls: 1.0 µL droplet acetone
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Acetone
- Concentration of vehicle in test medium (stock solution and final test solution): 100%
- Evaporation of vehicle before use: No

Oral
- Details of food source: 20% sucrose in water. A solution of the appropriate concentration of the test material in acetone (1 part) with 20% sucrose in water (19 parts) was used. The test material was administered as a single dose of 0.2 mL to each group of 10 bees.
- Method of feeding during study: Via glass tube 50 x 8 mm with a 1.5 mm opening. When the bees had taken all the test solution after 4 hours the dosage tubes were replaced by tubes containing 20% sucrose.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Acetone
- Concentration of vehicle in test medium (stock solution and final test solution): 100% stock solution. 1 part to 19 parts (5%) final test solution.
- Evaporation of vehicle before use: No details

Test organisms (species):

Apis mellifera

Animal group:

Hymenoptera (honeybees)

Details on test organisms:

TEST ORGANISM
- Common name: Honey bee
- Source: Mrs. P. Papworth, Four Winds, Houghton, Cambridgeshire
- Date of collection: 25 August 1987 for range-finding test
28 August 1987 for final test
- Cultural background (if honeybees): Sterile female workers
- Disease free: No details
- Kept according to standard practices: No details

ACCLIMATION
- Acclimation period: Bees dosed within 3 - 4 hours of removal from hive.
- Acclimation conditions (same as test or not): No details
- Feeding: 20% sucrose solution. For oral test bees not fed before dosing
- Health during acclimation (any mortality observed): No details

Study type:

laboratory study

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

No details

Test temperature:

24 ± 1ºC

pH (if soil or dung study):

Not applicable

Humidity:

Not reported

Photoperiod and lighting:

Total darkness except for essential procedures.

Details on test conditions:

TEST SYSTEM
- Test container (material, size): Cylindrical wire mesh cages 11.5 cm long x 4.0 cm in diameter
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 10
- No. of replicates per control: 2
- No. of replicates per vehicle control: 2

OTHER TEST CONDITIONS
- Photoperiod: Total darkness

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Mortality at 24 and 48 hours

VEHICLE CONTROL PERFORMED: yes

TEST CONCENTRATIONS
- Range finding study: Yes
- Test concentrations: 5 logarithmically spaced doses from 0.01 to 100 µg test material/bee
- Results used to determine the conditions for the definitive study: Yes. Limit test at 100 µg test material/bee

Nominal and measured concentrations:

Nominal concentrations: 5 logarithmically spaced doses from 0.01 to 100 µg/ bee used for range-finding dose. Final test used one nominal concentration of 100 µg test material/bee.

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

LD50

Effect conc.:

> 100 µg per animal

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: Result for both oral and contact routes.

Details on results:

Technical cloquintocet-mexyl exhibited low toxicity to bees with an LD50 greater than 100 µg/bee by both oral and contact routes.

Results with reference substance (positive control):

No positive control.

Reported statistics and error estimates:

Because only one concentration was tested, no dose-related response was obtained and no statistical analyses were required.

Validity criteria fulfilled:

yes

Conclusions:

Technical cloquintocet-mexyl exhibted low toxicity to honey bees (Apis mellifera) with an LD50 greater than 100 µg/bee by both oral and contact routes.

Executive summary:

In a GLP compliant study conducted to EPA FIFRA 71-2 test guideline, a 48 hour toxicity test was conducted with cloquintocet-mexyl to determine the acute oral and contact toxicity to honey bees (Apis mellifera). The LD50 was found to be greater than 100 µg/bee by both routes.

Description of key information

Oral and direct contact LD50 >100 µg/bee