Registration Dossier

Administrative data

Endpoint:
neurotoxicity
Remarks:
other: in vitro
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: irrelevant route of exposure (i.p.); only few experimental details

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
2003

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Mechanistic investigation of cholinesterase activity
GLP compliance:
no
Remarks:
: published study
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Phosphorus trichloride
EC Number:
231-749-3
EC Name:
Phosphorus trichloride
Cas Number:
7719-12-2
Molecular formula:
Cl3P
IUPAC Name:
lambda6-phosphorus(3+) trichloride
Details on test material:
Phosphorus oxychloride

Test animals

Species:
mouse
Strain:
Swiss Webster
Sex:
male

Administration / exposure

Doses / concentrations
Remarks:
Doses / Concentrations:
0, 10, 30, 100 mg/kg bw
Basis:
other: ip dose
Control animals:
yes, concurrent vehicle

Results and discussion

Any other information on results incl. tables

Serum (butyryl)cholinesterase, but not brain acetylcholinesterase was inhibited in vivo 1 h after exposure (ED50 (14 mg/kg bw). Mortality was seen at dose levels of 100 mg/kg bw and above.

Applicant's summary and conclusion

Conclusions:
No effects were seen on acetylcholinesterase activity in this study. Butyrylcholinesterase activity was inhibited at high dose levels.
Executive summary:

Intraperitoneal dosing of mice was found to result in the inhibition of butyrylcholinesterase activity at high dose levels, but did not affect cholinesterase activities. The effects are not of clear physiological relevance.