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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-GLP, near guideline study, published study report, fully adequate for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1986

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
GLP compliance:
not specified
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): tetrachloroethylene- Analytical purity: 99.9%- Lot/batch No.: TA03116F-01 - Stability under test conditions: yes. Tetrachloroethylene was found to be stable for 2 weeks at 60ºC- Storage condition of test material: stored at 0ºC

Test animals

Species:
rat
Strain:
Fischer 344
Sex:
male/female
Details on test animals and environmental conditions:
TEST ANIMALS- Source: Frederick Cancer Research Center- Age at study initiation: 5-7 weeks- Housing: stainless steel mesh, 1 animal per cage- Diet: ad libitum, except during inhalation exposure- Water: ad libitum, also during inhalation exposure- Acclimation period: 7 daysENVIRONMENTAL CONDITIONSdata not reported

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
other: unchanged (no vehicle)
Details on inhalation exposure:
GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTIONTetrachloroethylene vapor generated by bubbling clean, dry air (-40ºC dewpoint) through all glass impingers that contained tetrachloroethylene; the different concentrations obtained by varying the amount of air that was passed through the test material.
Analytical verification of test atmosphere concentrations:
not specified
Duration of exposure:
4 h
Concentrations:
2445, 3786, 4092, 4513 or 5163 ppm
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
Animals were observed daily and weighed on days 0 and 15. Necropsy was performed on all animals.
Statistics:
not reported

Results and discussion

Effect levels
Key result
Sex:
male/female
Dose descriptor:
other: lowest concentration causing mortality
Effect level:
3 786 ppm
Exp. duration:
4 h
Mortality:
All the rats that were exposed to 5163 ppm died before the end of the study, and deaths also occurred in all but the lowest dose groups.Survival: 5/5, 4/5, 3/5, 3/5, 0/5 (males) and 5/5, 1/5, 2/5, 2/5, 0/5 (females) in dose groups 2445, 3786, 4092, 4513, and 5163 ppm respectively.
Clinical signs:
Hypoactivity, ataxia, and anesthesia were observed in all dosed groups.
Body weight:
Mean body weight gain was not dose related.
Gross pathology:
No effects were reported.

Applicant's summary and conclusion