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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed according to the guideline.
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
Stock solution was prepared by dissolving 500 mg of the test substance in 1 liter of potable water (passed through reverse osmosis system) with 24 hrs of continuous stirring. After filteration water solubility obtained was 150 mg/L. From this stock solution, further test concentration was prepared for achieving test concentrations of 0.1 mg/L, 0.2 mg/L, 0.4 mg/L, 0.8 mg/L & 1.6 mg/L, respectively.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebra Fish
- Strain: Danio rerio
- Age of chemical: 3-4 months
- Length at study initiation (length definition, mean, range and SD): average 2.5 cm
- Weight at study initiation (mean and range, SD): 0.272 g (average)
- Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
- Duration of housing: 15 days
- Photoperiod: 12 hours light and 12 hours darkness
- Diet : Standard brand feed, once daily
- Acclimatization: seven days
- Feeding frequency: Once in a day
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Test temperature:
23°C
Dissolved oxygen:
7.5 mg/L
Nominal and measured concentrations:
Nominal concentrations: 0, 0.1, 0, 2, 0.4, 0.8 and 1.6 mg/l (nominal concentrations)
Details on test conditions:
TEST SYSTEM
- Test vessel: Aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 4 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 12 hours light and 12-hour darkness
Reference substance (positive control):
no
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 1.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
1.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality

Table 1: Mortality and symptoms:

NominalConcentration

(mg/l)

Number of fish

Mortality (cumulated) after

24h

48h

72h

96h

0 (control)

8

-

1

-

-

0.1

8

-

-

-

-

0.2

8

-

-

-

-

0.4

8

-

-

1

-

0.8

8

-

-

-

-

1.6

8

-

-

-

-

Visible Symptoms: Fishes were moving freely.

NominalConcentration(mg/l)

Number of fish

Symptoms after

24h

48h

72h

96h

0 (control)

8

NS8

NS7

NS7

NS7

0.1

8

NS8

NS8

NS8

NS8

0.2

8

NS8

NS8

NS8

NS8

0.4

8

NS8

NS8

NS7

NS7

0.8

8

NS8

NS8

NS8

NS8

1.6

8

NS8

NS8

NS8

NS8

Explanation of symptoms:

A: apathy

N: narcotic-like state

C: swimming near water surface

D: swimming at the bottom of bowl

M: Slow movements compared to control

NS: No abnormal symptoms

Number behind symbol for symptom= number of affected fish

Table 2. pH and oxygen content:

NominalConcentration(mg/l)

pH readings after

24h

48h

72h

96h

0 (control)

7.8

7.6

7.6

7.6

0.1

7.8

7.5

7.5

7.5

0.2

7.7

7.7

7.7

7.6

0.4

7.7

7.6

7.6

7.5

0.8

7.6

7.6

7.6

7.4

1.6

7.6

7.6

7.4

7.4

NominalConcentration(mg/l)

Oxygen content (mg/l) after

24h

48h

72h

96h

0 (control)

7.2

7.0

6.9

6.9

0.1

7.1

7.0

6.9

6.8

0.2

7.1

6.9

6.8

6.8

0.4

7.2

7.0

6.9

6.8

0.8

7.3

7.2

7.1

6.7

1.6

7.3

7.2

7.0

6.5

Table 3. Test temperature

NominalConcentration(mg/l)

Temperature (°C) after

24h

48h

72h

96h

0 (control)

21

22

23

23

0.1

21

22

23

23

0.2

21

22

23

23

0.4

21

22

23

23

0.8

21

22

23

23

1.6

21

22

23

23

Conclusions:
Based on nominal concentrations, the median lethal concentrations [LC50 (96 h)] for test chemical on Danio rerio (Zebra Fish) was determined to be > 1.6 mg/L.
Executive summary:

Short term toxicity to fish was conducted for 96 hrs for assessing the effect of test chemical. The test was performed in accordance to OECD guideline No. 203 “Fish Acute Toxicity Test”. Zebra fish (Danio rerio) of average weight 0.272 g and average length of 2.5 cm was used as a test organism for the study. Test fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration. During the housing period, test fishes were fed once daily with standard brand fed. The test conditions during the housing of the test organisms were oxygen content of 7.4 mg/l, pH 7.3, water temperature 24°C and under a photoperiod of 12:12 hr light: dark conditions, respectively. The limit test (100 mg/l) was conducted for this substance. Thus, on the basis of this limit test, definitive test was performed at different test chemical nominal concentrations, i.e., at 0, 0.1, 0.2, 0.4, 0.8 and 1.6 mg/l (nominal concentrations), respectively. Total 8 fishes were exposed to test chemical in a 5 lit bowl aquaria containing 4 liters of potable water. The test vessels were placed in a room at a temperature of 23°C, pH of 6.9, hardness of water 46.25 mg of CaCO3 and under a photoperiod of 12:12 hr light: dark conditions, respectively. Aeration in test vessels was provided 1 day before the start of the experiment. Test fishes were moving slowly as compared to control. On the basis of effect of test chemical on mortality of the test organism, the LC0 and median lethal concentration (LC50 (96 h)) value was determined to be 1.6 mg/l and > 1.6 mg/l, respectively.

Description of key information

Short term toxicity to fish was conducted for 96 hrs for assessing the effect of test chemical. The test was performed in accordance to OECD guideline No. 203 “Fish Acute Toxicity Test” (Study report, 2015). Zebra fish (Danio rerio) of average weight 0.272 g and average length of 2.5 cm was used as a test organism for the study. Test fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration. During the housing period, test fishes were fed once daily with standard brand fed. The test conditions during the housing of the test organisms were oxygen content of 7.4 mg/l, pH 7.3, water temperature 24°C and under a photoperiod of 12:12 hr light: dark conditions, respectively. The limit test (100 mg/l) was conducted for this substance. Thus, on the basis of this limit test, definitive test was performed at different test chemical nominal concentrations, i.e., at 0, 0.1, 0.2, 0.4, 0.8 and 1.6 mg/l (nominal concentrations), respectively. Total 8 fishes were exposed to test chemical in a 5 lit bowl aquaria containing 4 liters of potable water. The test vessels were placed in a room at a temperature of 23°C, pH of 6.9, hardness of water 46.25 mg of CaCO3 and under a photoperiod of 12:12 hr light: dark conditions, respectively. Aeration in test vessels was provided 1 day before the start of the experiment. Test fishes were moving slowly as compared to control. On the basis of effect of test chemical on mortality of the test organism, the LC0 and median lethal concentration (LC50 (96 h)) value was determined to be 1.6 mg/l and > 1.6 mg/l, respectively.

Key value for chemical safety assessment

LC50 for freshwater fish:
1.6 mg/L

Additional information

Short term toxicity to fish was conducted for 96 hrs for assessing the effect of test chemical. The test was performed in accordance to OECD guideline No. 203 “Fish Acute Toxicity Test” (Study report, 2015). Zebra fish (Danio rerio) of average weight 0.272 g and average length of 2.5 cm was used as a test organism for the study. Test fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration. During the housing period, test fishes were fed once daily with standard brand fed. The test conditions during the housing of the test organisms were oxygen content of 7.4 mg/l, pH 7.3, water temperature 24°C and under a photoperiod of 12:12 hr light: dark conditions, respectively. The limit test (100 mg/l) was conducted for this substance. Thus, on the basis of this limit test, definitive test was performed at different test chemical nominal concentrations, i.e., at 0, 0.1, 0.2, 0.4, 0.8 and 1.6 mg/l (nominal concentrations), respectively. Total 8 fishes were exposed to test chemical in a 5 lit bowl aquaria containing 4 liters of potable water. The test vessels were placed in a room at a temperature of 23°C, pH of 6.9, hardness of water 46.25 mg of CaCO3 and under a photoperiod of 12:12 hr light: dark conditions, respectively. Aeration in test vessels was provided 1 day before the start of the experiment. Test fishes were moving slowly as compared to control. On the basis of effect of test chemical on mortality of the test organism, the LC0 and median lethal concentration (LC50 (96 h)) value was determined to be 1.6 mg/l and > 1.6 mg/l, respectively.