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Ecotoxicological information

Toxicity to microorganisms

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Reference
Endpoint:
toxicity to microorganisms, other
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
experimental data of read across substances
Justification for type of information:
Data for the target chemical is summarized based on the structurally similar read across chemicals
Reason / purpose:
read-across source
Reason / purpose:
read-across source
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
WoE report is based on two toxicity to microorganism studies as-
WoE 2. and WoE 3.
GLP compliance:
not specified
Analytical monitoring:
not specified
Vehicle:
not specified
Details on test solutions:
WoE 2: Test compound dissolved in distilled water; osmolality adjusted to 2 % NaCl.
WoE 3: no data available
Test organisms (species):
other: WoE 2: V. fischeri and WoE 3: Bacillus subtilis
Details on inoculum:
WoE 2:
- Laboratory culture: Bacteria V. fischeri (NRRL B-1117) was supplied as freeze-dried reagent, BioFix Lumi, from Macherey-Nagel, Du¨ ren, Germany

WoE 3: no data available
Test type:
not specified
Water media type:
not specified
Total exposure duration:
24 h
Remarks on exposure duration:
WoE 2: 30 mins and WoE 3: 24 hr
Details on test conditions:
WoE 2: EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Light production from luminescent bacteria was measured with a luminometer equipped with a constant
temperature water bath (15°C).

WoE 3: no data available
Reference substance (positive control):
not specified
Duration:
30 min
Dose descriptor:
EC50
Effect conc.:
> 39.9 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other:
Remarks on result:
other: WoE 2
Dose descriptor:
EC50
Effect conc.:
0.53 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Remarks on result:
other: WoE 3: EC50 reported to be 0.0013 mM/l = 0.53 mg/l
Validity criteria fulfilled:
not specified
Conclusions:
On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach, the EC50 value of the test chemical on test organism can be expected to be in the range of 0.53 to > 39.9 mg/l.
Executive summary:

Data available for its structurally similar read across chemicals has been reviewed to determine the toxic effect of the test chemical on microorganisms.The studies are as mentioned below:

Toxicity to microorganisms study was carried out for 30 mins. The study was performed in accordance with the ISO 11348-2 guideline. Bacteria V. fischeri (NRRL B-1117) was supplied as freeze-dried reagent, BioFix Lumi, from Macherey-Nagel, Du¨ ren, Germany. Test chemcal was dissolved in distilled water; osmolality adjusted to 2 % NaCl. Test chemical concentration used for the study was <=39.9 mg/L. Light production from luminescent bacteria was measured with a luminometer equipped with a constant temperature water bath (15°C). On the basis of the effect of test chemical on light production of the test organism V. fischeri, the 30 mins EC50 value was determined to be > 39.9 mg/l.

Another toxicity to micro-organism study was carried out using Bacillus subtilis as test organism. On the basis on effect of test chemical on growth rate of the test organism, the EC50 value was determined to be 0.53 mg/l.

On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach, the EC50 value of the test chemical on test organism can be expected to be in the range of 0.53 to > 39.9 mg/l.

Description of key information

On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach, the EC50 value of the test chemical on test organism can be expected to be in the range of 0.53 to > 39.9 mg/l.

Key value for chemical safety assessment

EC50 for microorganisms:
0.53 mg/L

Additional information

Data available for its structurally similar read across chemicals has been reviewed to determine the toxic effect of the test chemical on microorganisms. The studies are as mentioned below:

 

Toxicity to microorganisms study was carried out for 30 mins. The study was performed in accordance with the ISO 11348-2 guideline. Bacteria V. fischeri (NRRL B-1117) was supplied as freeze-dried reagent, BioFix Lumi, from Macherey-Nagel, Du¨ ren, Germany. Test chemcal was dissolved in distilled water; osmolality adjusted to 2 % NaCl. Test chemical concentration used for the study was <=39.9 mg/L. Light production from luminescent bacteria was measured with a luminometer equipped with a constant temperature water bath (15°C). On the basis of the effect of test chemical on light production of the test organism V. fischeri, the 30 mins EC50 value was determined to be > 39.9 mg/l.

 

Another toxicity to micro-organism study was carried out using Bacillus subtilis as test organism. On the basis on effect of test chemical on growth rate of the test organism, the EC50 value was determined to be 0.53 mg/l.

 

On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach, the EC50 value of the test chemical on test organism can be expected to be in the range of 0.53 to > 39.9 mg/l.