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EC number: 237-158-7 | CAS number: 13674-84-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
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- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
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- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
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- Specific investigations
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- Additional toxicological data

Additional ecotoxological information
Administrative data
- Endpoint:
- additional ecotoxicological information
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study was published in reliable scientific journal. The test course was detailed introduced, so the reliability can be defined as reliable with restrictions.
Data source
Reference
- Reference Type:
- publication
- Title:
- Investigation of cytotoxic, genotoxic, mutagenic, and estrogenic effects of the flame retardants tris-(2-chloroethyl)-phosphate (TCEP) and tris-(2-chloropropyl)-phosphate (TCPP) in vitro
- Author:
- W. Follmann, J. Wober
- Year:
- 2 005
- Bibliographic source:
- Toxicology Letters 161 (2006) 124–134
Materials and methods
Test guideline
- Qualifier:
- no guideline required
- Principles of method if other than guideline:
- using the recombinant yeast reporter gene assay and by induction of the alkaline phosphatase enzyme in human endometrial cancer Ishikawa cells.
- GLP compliance:
- not specified
- Type of study / information:
- Endocrine disrupting effects
Test material
- Reference substance name:
- Tris(2-chloro-1-methylethyl) phosphate
- EC Number:
- 237-158-7
- EC Name:
- Tris(2-chloro-1-methylethyl) phosphate
- Cas Number:
- 13674-84-5
- Molecular formula:
- C9H18Cl3O4P
- IUPAC Name:
- tris(2-chloro-1-methylethyl) phosphate
- Details on test material:
- Testing substance was the same with the substance notified in the section 1 of this dossier. tris-(2-chloropropyl)- phosphate (TCPP) (CAS No.: 13674-84-5) was from Akzo-Nobel (Deventer, Netherlands). insulin-transferrin-seleniumA-supplement, DMEM and DMEM/F12 culture medium were from Invitrogen (Eggenstein, Germany), and fetal calf serum from Biochrom (Berlin, Germany). Chlorphenol-red-β-dgalactopyranoside (CRPG) and p-nitrophenylphosphate (NPP) were from Roche (Mannheim, Germany). Sterile plastic materials were from Greiner (Solingen, Germany).
Constituent 1
Results and discussion
Any other information on results incl. tables
Induction of the activity of the enzyme alkaline phosphatase in Ishikawa cells (human endometrial adenocarcinoma cell line) is seen as a hint for an estrogenic potential of a test substance. TCPP did not induce activity of alkaline phosphatase in contrast to the positive control (estradiol 1μM).
To confirm the latter results in further screening system estrogen receptor-mediated effects were examined with the Recombinant Yeast Assay with GLAXO ERα yeasts. In this assay an induction ofactivity of β-galactosidase secreted from the GLAXO ERα yeasts demonstrates an estrogenic effect of a test substance. TCPP did not induceβ-galactosidase activity whereas for the positive control (estradiol) clear induction was measured. To test vice versa the antiestrogenic potential of TCPP and TCEP,GLAXO ERα yeasts were induced by estradiol and then treated with the test substances. As demonstrated in, TCPP did not reduce the induction origination from estradiol indicating that both have no antiestrogenic potential.
Applicant's summary and conclusion
- Conclusions:
- This study was used an advanced method to dig out the oestrogenic or anti-oestrogenic effects of TCPP, so the results were fulfilled all validity criteria.
- Executive summary:
Oestrogenic/anti-oestrogenic effects have been investigated by Föllmann and Wober (2006) using the recombinant yeast reporter gene assay and by induction of the alkaline phosphatase enzyme in human endometrial cancer Ishikawa cells. This study was used an advanced method to dig out the oestrogenic or anti-oestrogenic effects of TCPP, so the results were fulfilled all validity criteria. No induction of oestrogenic or anti-oestrogenic effects was detected in either of the test systems.
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