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Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Additional information

Study reports have been available for consideration in respect of acute tests with fish, invertebrates, algae and micro-organisms and acute and chronic tests with aquatic invertebrates. The contents of the test reports are summarised below table.

Test species

Test protocol

Year test completed

Endpoint and exposure period

Result (mg/l)1

Reliability assessment

Comments

Study reference

Toxicity to fish

Zebrafish (Brachydanio rerio)

Not given

1991

96-h LC0

24-h LC50

48-h LC50

72-h LC50

96-h LC50

32

56

56

56

56

(1) valid without restriction

Fulfils all reliability criteria. Static test. Test results are presented as the geometric mean of 24, 48, 72 and 96-h LC0and LC100values. LC50values are therefore approximate

Kanne, 1991

 

Bluegill Sunfish (Lepomis macrochirus)

Not given

1985

96-h NOEC

96-h LC50

6.3

84

(1) valid without restriction

Static test. The 96-h LC50was determined by extrapolation outside the range of test concentrations. Comparison with a reported 120-h LC50value of approximately 20 mg/l (based on mean measured concentrations) suggests that the 96 hour value is reasonable although perhaps a little high.

Meeks, 1985a

 

 

Fathead Minnows (Pimephales promelas)

Not given

1985

96-h NOEC

24-h LC50

48-h LC50

72-h LC50

96-h LC50

9.8

>51

>51

51

51

(1) valid without restriction

 

 

Fulfils all reliability criteria. Static test but with analysis of exposure concentrations. 

Meeks, 1985b

 

 

 

 

 

 

 

Guppy (Poecilia reticulata)

OECD 203; ISO 7346-1

1997

48-h LC20

48-h LC50

96-h LC20

96-h LC50

22 (N*)

40 (N)

17 (N)

30 (N)

(4) not assignable

Static test. Only a summary report was available for review and there is insufficient information to fully evaluate the standard of the test. The test was not supported by chemical analysis and was not subject to GLP.

Griebenow, 1998

Killifish (Oryzias latipes)

Japanese Industrial Standard (JIS K 0102-1986-71)

Not given

48-h LC50

54 (N)

(4) not assignable

Data are from a secondary source. Given that the data are not critical for deriving the PNEC the original test report has not been reviewed.

MITI, 1992

Fish – acute QSAR (Esters)

ECOSAR (version

0.99g)

 

96-h LC50

21

 

 

 

 

The estimated values are of the same order as the

measured values.

The estimates were obtained using measured physicochemical data as inputs to the model.

 

 

 

 

 

 

Fish – acute QSAR (Phosphate esters)

ECOSAR (version 0.99g)

 

96-h LC50

11

 

 

 

 

Fish – chronic QSAR (Esters)

ECOSAR (version 0.99g)

 

NOEC

5.2

 

 

 

 

Toxicity to aquatic invertebrates

 

Cladoceran (Daphnia magna)

DIN 38412/L11

1997

24-h EC20

24-h EC50

48-h EC20

48-h EC50

57 (N)

75 (N)

51 (N)

63 (N)

(4) not

assignable

 

 

Only a summary report was available for review and

there is insufficient information to fully evaluate the standard of the test. The test was not supported by

chemical analysis and was not subject to GLP.

 

Griebenow,

 

1998

 

 

 

 

 

Cladoceran (Daphnia magna)

Not given

1985

48-h NOEC

48-h EC50

33.5

131

(1) valid without restriction

Fulfils all reliability criteria.

Meeks, 1985c

Invertebrate - acute QSAR(Esters)

ECOSAR (version

0.99g)

 

48-h LC50

 

 

63

 

 

 

 

 

The estimated value is of the same order as the

measured values.

The estimates were obtained using measured physicochemical data as inputs to the model.

 

 

 

Cladoceran (Daphnia magna)

OECD 202

1995

21-day NOEC (parent mortality)

32 (N)

(1) valid

Fulfils all reliability criteria. Analysis of exposure

Sewell,

 

without

concentrations confirmed that they were close to

Foulger and

21-day NOEC (reproduction)

32 (N)

restriction

nominal. Results are therefore expressed relative to nominal.

Bartlett, 1995

14-day EC50(parent immobilisation)

42 (N)

 

 

 

21-day EC50(parent immobilisation)

40 (N)

 

 

 

21-day EC50(reproduction)

32-56 (N)

 

 

 

Invertebrate – longer term repro QSAR (Neutral organics)

ECOSAR (version 0.99h)

 

16-d EC50(reproduction)

4.3

 

A recommended valid QSAR method is not readily available for the endpoint of chronic invertebrate. The method used, while the most appropriate from ECOSAR for this substance, is not recommended by ECOSAR for this type of compound and the QSAR is not well validated.

 

However the estimated value is within an order of magnitude of the measured value. The estimate was obtained using measured physicochemical data as inputs to the model.

 

Toxicity to algae

 

 

Freshwater alga (Pseudokirchneriella subcapitata)

OECD 201; EEC Dir 92/69/EEC, Method C3

2004

72-h NOEC

72-h ErC10(growth)

72-h EbC10(biomass) 72-h ErC50(growth)

72-h EbC50(biomass)

13 (N)

42 (N)

14 (N)

82 (N)

33 (N)

(1) valid without restriction

Fulfils all the reliability criteria. Results are expressed relative to nominal concentrations because measured concentrations were within 80-120% of nominal. The study was subject to GLP.

Desjardins (2004)

 

 

Freshwater alga (subspicatus

Scenedesmus)

DIN 38412/L33

1997

72-h EC20

72-h EC50

25 (N)

45 (N)

(4) not assignable

Only a summary report was available for review and there is insufficient information to fully evaluate the standard of the test. The test was not supported by chemical analysis and was not subject to GLP.

Griebenow, 1998

 

 

Freshwater alga (Selenastrum capricornutum) – note: now known asPseudokirchneriella subcapitata

OECD 201; EEC DOC 89/88/XI, Directive 79/831, Annex V-C3

1991

96-h NOEC

96-h LOEC

96-h ErC50(growth)

96-h EbC50(biomass)

6 (N)

18 (N)

73 (N)

47 (N)

(3) invalid

The test was not supported by chemical analysis and test media were prepared by dilution of a stock suspension. These are significant inadequacies that invalidate the data for the purposes of risk assessment.

Kroon and van Ginkel, 1992

 

 

Algae QSAR (Esters)

ECOSAR (version 0.99g)

 

96-h EC50

96-h NOEC

1.8

1.4

 

The estimated values are lower than the measured values. The estimates were obtained using measured physicochemical data as inputs to the model.

 

 

 

Toxicity to micro-organisms

 

 

Activated sludge

ISO 8192

 

EC50

784

(2) valid with restrictions

The test is of an overall acceptable standard, despite some limitations in the test report.

Bayer, 1990

 

 

Photobacterium (Vibrio fischeri)

 

 

15-minute EC50

171.5 ppm (95% confidence interval of 149.0 – 197.0 ppm)

(3) invalid

Result of a LUMIStox bacteriological assay as part of a monitoring study from the open literature. The organism is also known as Photobacterium

Guzzella and Galassi, 1993

 

 

Note:

1‘N’ denotes result expressed as nominal concentration