Registration Dossier

Diss Factsheets

Administrative data

Description of key information

The substance sodium 2-(2-dodecyloxyethoxy) ethyl sulphate found to be irritating to the skin and eye 

Key value for chemical safety assessment

Skin irritation / corrosion

Link to relevant study records
Reference
Endpoint:
skin irritation: in vivo
Type of information:
(Q)SAR
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Justification for type of information:
QSAR prediction: migrated from IUCLID 5.6
Qualifier:
according to guideline
Guideline:
other: estimated data
Principles of method if other than guideline:
Prediction is done using QSAR Toolbox version 3.1
GLP compliance:
no
Species:
rabbit
Strain:
not specified
Type of coverage:
occlusive
Preparation of test site:
not specified
Vehicle:
not specified
Controls:
not specified
Duration of treatment / exposure:
Predicte data
Number of animals:
5-6
Irritation parameter:
other: Primary irritation index (PII)
Basis:
mean
Score:
2.87
Reversibility:
other: Estimated
Remarks on result:
other: irritating to skin of rabbit

The prediction was based on dataset comprised from the following descriptors: "Primary Irritation Index"
Estimation method: Takes average value from the 5 nearest neighbours
Domain  logical expression:Result: In Domain

((("a" or "b" or "c" or "d" or "e" or "f" )  and "g" )  and ("h" and "i" )  )

Domain logical expression index: "a"

Referential boundary: The target chemical should be classified as Alkoxy AND Ether AND Sulfate by Organic functional groups

Domain logical expression index: "b"

Referential boundary: The target chemical should be classified as Alkoxy AND Ether AND Sulfate by Organic functional groups (nested)

Domain logical expression index: "c"

Referential boundary: The target chemical should be classified as Aliphatic Carbon [CH] AND Aliphatic Carbon [-CH2-] AND Aliphatic Carbon [-CH3] AND Miscellaneous sulfide (=S) or oxide (=O) AND Oxygen, aliphatic attach [-O-] AND Suflur {v+4} or {v+6} AND Sulfate, linear [-O-SO2-O-] AND Sulfite, linear [-OS(=O)O-] by Organic functional groups (US EPA)

Domain logical expression index: "d"

Referential boundary: The target chemical should be classified as Anion AND Cation AND Dialkylether AND Ether AND Sulfuric acid derivative by Organic functional groups, Norbert Haider (checkmol)

Domain logical expression index: "e"

Referential boundary: The target chemical should be classified as Group 1 - Alkali Earth Li,Na,K,Rb,Cs,Fr AND Group 14 - Carbon C AND Group 16 - Oxygen O AND Group 16 - Sulfur S by Chemical elements

Domain logical expression index: "f"

Referential boundary: The target chemical should be classified as Alkali Earth by Groups of elements

Domain logical expression index: "g"

Referential boundary: The target chemical should be classified as Bioavailable by Lipinski Rule Oasis

Domain logical expression index: "h"

Parametric boundary:The target chemical should have a value of log Kow which is >= -1.86

Domain logical expression index: "i"

Parametric boundary:The target chemical should have a value of log Kow which is <= 1.7

Interpretation of results:
irritating
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
By primary irritation index (PII) from QSAR, skin irritation score of sodium 2-(2-dodecyloxyethoxy) ethyl sulphate estimated as 2.87.Based upon this score it is indicated that sodium 2-(2-dodecyloxyethoxy) ethyl sulphate is irritating to the skin of rabbit.
Executive summary:

By primary irritation index (PII) from QSAR, skin irritation score of sodium 2-(2-dodecyloxyethoxy) ethyl sulphate estimated as 2.87.Based upon this score it is indicated that sodium 2-(2-dodecyloxyethoxy) ethyl sulphate is irritating to the skin of rabbit.

Endpoint conclusion
Endpoint conclusion:
adverse effect observed (irritating)

Eye irritation

Link to relevant study records
Reference
Endpoint:
eye irritation: in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Qualifier:
according to guideline
Guideline:
other:
Principles of method if other than guideline:
The pollen tube growth test (PTG test) based on the growth response of in vitro growing tobacco pollen tubes were carried out for studiying ED50 value. the PTG test was among the five best of 30 in vitro alternatives to the Draize primary eye irritation test
GLP compliance:
not specified
Species:
other: Nicotiana
Strain:
other: Nicotiana sylvestr
Details on test animals or tissues and environmental conditions:
Test Plant:
Tobacco plants of the species Nicotiana sylvestr (Spegazz& Comesvar. SCR) were cultivated from seeds in a greenhouse and set out to flower in the Botanical Garden of the University of Hamburg.The anthers of flowers that were not yet fully open were harvested and dried for two days at 20 deg C in a Petridish.The pollen was obtained by shaking the dried anthers and then freezing them in Eppendorf tubes (1.5 ml) at minus 18degC.

Growth Medium:
The culture mediumis composed of 10% saccharos(w/v), 0.01% boric acid( w/v), and 3 mM Ca(NO32) in double-distilled water.It is buffered w ith 10 mM MES( 2-[N-morpholino]-ethanesulfonaicc id) and adjusted to a pH of 5.6 with KOH.
Vehicle:
other: DMSO
Controls:
yes
Amount / concentration applied:
10mM to 10-5 mM
Duration of treatment / exposure:
18 hrs
Observation period (in vivo):
18 hrs
Irritation parameter:
other: ED50
Time point:
other: 18 hrs
Score:
8.87
Reversibility:
not specified
Interpretation of results:
irritating
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
The ED50 value of sodium 2-(2-dodecyloxyethoxy) ethyl sulphate was found to be 8.87 ppm. Based on in vivo data the substance 2-(2-dodecyloxyethoxy) ethyl sulphate was classified as strong irritant to eye
Executive summary:
Frozen pollen grain were resuspende in culture medium at a concentration of 1mg/ml. Test substances were serially diluted by a factor of 2. Dilutions were transferred the pollen suspension and incubated for18 h at 25 degC. Pollen tube cultures were washed twice with double distilled water and then stained with Alcian blue (0.05%) for 30 min. The dyed pollen tube walls were washed and finally destaine with citric acid (40%). The optical density was measured at 607nm
Each concentration was tested in quadruplicates. The ED50 value of sodium 2-(2-dodecyloxyethoxy) ethyl sulphate was found to be 8.87 ppm. Based on in vivo data the substance 2-(2-dodecyloxyethoxy) ethyl sulphate was classified as strong irritant to eye
According to Kristen et.al.(1992). The ED50 value of sodium 2-(2-dodecyloxyethoxy) ethyl sulphate was found to be 8.87 ppm. Based on in vivo data the substance 2-(2-dodecyloxyethoxy) ethyl sulphate was classified as strong irritant to eye
Endpoint conclusion
Endpoint conclusion:
adverse effect observed (irritating)

Respiratory irritation

Endpoint conclusion
Endpoint conclusion:
no study available

Additional information

Skin irritation:-

Based on studies of target substance CAS NO 3088-31-1 and read across reviewed for skin irritation from reliable sources having Klimisch rating 2 and 4 considering weight of evidence approach.

The summary of the results are presented below

 

Sr. No

End point

effect

Species

Remark

1

Overall irritation score

irritating

Rabbits

Predicted data of target chemical

2

Overall irritation score

irritating

Rabbits

Study report for target chemical

3

Overall irritation score

mild irritating

Rabbits

Data from publication for CAS: 151-21-3

4

Overall irritation score

mild irritating

mouse

Data from publication for CAS: 151-21-3

5

Overall irritation score

Slightly irritating

piglet

Data from publication for CAS: 151-21-3

6

Overall irritation score

not irritating

Guinea pig

Data from publication for CAS: 151-21-3

7

Overall irritation score

not irritating

pig

Data from publication for CAS: 151-21-3

 

Based on results summarized in above table for the target chemical CAS NO 3088-31-1 and read across, it can be concluded that the substance is irritating to the skin of Rabbit and mouse. ThusSodium 2-(2-dodecyloxyethoxy) ethyl sulphateis considered to be skin irritant as the criteria of CLP regulation and classified as skin irri category 2.

Eye irritation:-

Based on studies of target substance CAS NO 3088-31-1 and read across reviewed for eye irritation from reliable sources having Klimisch rating 2 and 4considering weight of evidence approach

 The summary of the results are presented below

 

Sr. No

End point

effect

Species

Remark

1

ED50

 

irritating

Nicotiana sylvestr

Data from publication for target chemical

2

MMAS(modified maximum average score (MMAS))

irritating

Rabbit

Predicted data for target chemical

 

3

Overall irritation score

irritating

Rabbit

Study report for target chemical

4

ED50

 

irritating

Nicotiana sylvestr

Data from publication for CAS NO 151-21-3

 

Based on the results summarized in above tables for the target chemical CAS NO 3088-31-1 and read across, it can be concluded that the substance is irritating to the eye of rabbit andNicotiana sylvestr.Thus based on this results Sodium 2-(2-dodecyloxyethoxy) ethyl sulphate is considered to be eye irritant as the criteria for CLP regulation and classified as eye irri category 2.  


Justification for selection of skin irritation / corrosion endpoint:
By primary irritation index (PII) from QSAR, skin irritation score of sodium 2-(2-dodecyloxyethoxy) ethyl sulphate estimated as 2.87.Based upon this score it is indicated that sodium 2-(2-dodecyloxyethoxy) ethyl sulphate is irritating to the skin of rabbit.

Justification for selection of eye irritation endpoint:
Frozen pollen grain were resuspende in culture medium at a concentration of 1mg/ml. Test substances were serially diluted by a factor of 2. Dilutions were transferred the pollen suspension and incubated for18 h at 25 degC. Pollen tube cultures were washed twice with double distilled water and then stained with Alcian blue (0.05%) for 30 min. The dyed pollen tube walls were washed and finally destaine with citric acid (40%). The optical density was measured at 607nm
Each concentration was tested in quadruplicates. The ED50 value of sodium 2-(2-dodecyloxyethoxy) ethyl sulphate was found to be 8.87 ppm. Based on in vivo data the substance 2-(2-dodecyloxyethoxy) ethyl sulphate was classified as strong irritant to eye

Effects on skin irritation/corrosion: irritating

Effects on eye irritation: irritating

Justification for classification or non-classification

The available studies indicate that the substance sodium 2-(2-dodecyloxyethoxy) ethyl sulphate is classified as a skin and eye irritant according to CLP regulation.

Categories Display