Sodium 2-(2-dodecyloxyethoxy)ethyl sulphate

Eye irritation

Administrative data

Endpoint:

eye irritation: in vitro / ex vivo

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

publication

Title:

The pollen tube growth test: A new alternative to the Draize eye irritation assay

Author:

UDO KRISTEN, UDO HOPPE, and WOLFGANG PAPE,

Year:

1993

Bibliographic source:

j. Soc.Cosmet.Chem.,44, 153-162 (1993)

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other:

Principles of method if other than guideline:

The pollen tube growth test (PTG test) based on the growth response of in vitro growing tobacco pollen tubes were carried out for studiying ED50 value. the PTG test was among the five best of 30 in vitro alternatives to the Draize primary eye irritation test

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Sodium 2-(2-dodecyloxyethoxy)ethyl sulphate

EC Number:

221-416-0

EC Name:

Sodium 2-(2-dodecyloxyethoxy)ethyl sulphate

Cas Number:

3088-31-1

Molecular formula:

C16H34O6S.Na

IUPAC Name:

sodium 2-(2-dodecyloxyethoxy)ethyl sulphate

Details on test material:

- Name of test material (as cited in study report): sodium 2-(2-dodecyloxyethoxy) ethyl sulphate
- Substance type: Organic
- Physical state: liquid

Test animals / tissue source

Species:

other: Nicotiana

Strain:

other: Nicotiana sylvestr

Details on test animals or tissues and environmental conditions:

Test Plant:
Tobacco plants of the species Nicotiana sylvestr (Spegazz& Comesvar. SCR) were cultivated from seeds in a greenhouse and set out to flower in the Botanical Garden of the University of Hamburg.The anthers of flowers that were not yet fully open were harvested and dried for two days at 20 deg C in a Petridish.The pollen was obtained by shaking the dried anthers and then freezing them in Eppendorf tubes (1.5 ml) at minus 18degC.

Growth Medium:
The culture mediumis composed of 10% saccharos(w/v), 0.01% boric acid( w/v), and 3 mM Ca(NO32) in double-distilled water.It is buffered w ith 10 mM MES( 2-[N-morpholino]-ethanesulfonaicc id) and adjusted to a pH of 5.6 with KOH.

Test system

Vehicle:

other: DMSO

Controls:

yes

Amount / concentration applied:

10mM to 10-5 mM

Duration of treatment / exposure:

18 hrs

Observation period (in vivo):

18 hrs

Results and discussion

In vivo

Results

Irritation parameter:

other: ED50

Time point:

other: 18 hrs

Score:

8.87

Reversibility:

not specified

Applicant's summary and conclusion

Interpretation of results:

irritating

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The ED50 value of sodium 2-(2-dodecyloxyethoxy) ethyl sulphate was found to be 8.87 ppm. Based on in vivo data the substance 2-(2-dodecyloxyethoxy) ethyl sulphate was classified as strong irritant to eye

Executive summary:

Frozen pollen grain were resuspende in culture medium at a concentration of 1mg/ml. Test substances were serially diluted by a factor of 2. Dilutions were transferred the pollen suspension and incubated for18 h at 25 degC. Pollen tube cultures were washed twice with double distilled water and then stained with Alcian blue (0.05%) for 30 min. The dyed pollen tube walls were washed and finally destaine with citric acid (40%). The optical density was measured at 607nm

Each concentration was tested in quadruplicates. The ED50 value of sodium 2-(2-dodecyloxyethoxy) ethyl sulphate was found to be 8.87 ppm. Based on in vivo data the substance 2-(2-dodecyloxyethoxy) ethyl sulphate was classified as strong irritant to eye

According to Kristen et.al.(1992). The ED50 value of sodium 2-(2-dodecyloxyethoxy) ethyl sulphate was found to be 8.87 ppm. Based on in vivo data the substance 2-(2-dodecyloxyethoxy) ethyl sulphate was classified as strong irritant to eye