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EC number: 446-620-9
CAS number: 120983-72-4
This study was designed to assess the toxicity of the test substance to
bacteria. The study was conducted according to the Commission Directive
88/302/EEC; Official Journal of the EC L 133 Part C (1988). This test
method is in most parts identical with OECD Guideline 209.
The method assesses the effect of a test substance on microorganisms by
measuring the respiration rate after 3 h under defined conditions in the
presence of different concentrations of the test substance (1, 3.1, 10,
31, 100 mg/L). A physico-chemical oxygen consumption control is carried
out, since some substances·can also consume oxygen by chemical
reactivity. In order to be able to tell the difference between
physico-chemical oxygen consumption and biological oxygen consumption
(respiration), at least the maximum concentration of the test substance
is tested without activated sludge.
For the test substance a 3h-EC50 of 6.3 mg/L towards bacteria was
A toxicity of the reference substance 3,5 -Dichlorophenol of 3h-EC50:
8.3 mg/L was determined.
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