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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
not applicable
GLP compliance:
yes
Analytical monitoring:
yes
Remarks:
Gas Chromatography (GC)
Details on sampling:
Sampling schedule of chemical analysis
- Control: at O and 48 hours
- Test concentrations: at 0 and 48 hours at 0 and 48 hours
Vehicle:
no
Details on test solutions:
To produce the highest test concentration (1.2 mg/L) the test substance was added daily to 1 litre of dilution water, then treated with an ultra turrax for 60 seconds and afterwards stirred for 24 hours on a magnetic stirrer. Finally undissolved particles of the test substance were removed by filtration. The test concentrations 0.125, 0.25, and 0.5 mg/L were prepared daily by diluting a stock solution (0.5 mg/L) and treated in the same way as the test concentration 1 mg/L.
Test organisms (species):
Daphnia magna
Details on test organisms:
Name: Daphnia magna STRAUS, parthenogenetic females
Source: Strain ofBundesgesundheitsamt Berlin
Maintenance and Acclimatisation: A population of parthenogenetic females of synchronized age structure is maintained since more than 15 years in the test facility under constant temperature conditions (20 +/- 1 °C) at a 16 : 8 hour light-dark photoperiod (illumination: < 1000 lux). The culture water (so-called 'M4 medium') is partly renewed once a week. The Daphnia are exclusively fed with unicellular green algae (Desmodesmus subspicatus CHODAT) 'ad libitum'. Mortalities of parent Daphnia during the culture period are recorded daily in a semiquantitative way. The neonates are separated from their parent Daphnia by filtration prior to the acute test.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
15.0 °dH (= 267.8 mg/L CaCO3)
Test temperature:
19.8 - 19.9 °C
pH:
7.8 - 7.9
Dissolved oxygen:
7.6 - 8.6 mg/L (concerning the highest test concentration)
Salinity:
n.a.
Conductivity:
No data
Nominal and measured concentrations:
- Test concentrations (nominally): 0.125, 0.25, 0.5 and 1 mg/L
- Test concentrations (measured): in the range between 62.4 and 92% of nominal values in the freshly prepared medium and between 20 and 52% of nominal values after 24 hours of exposure
Details on test conditions:
Exposure conditions:
- Test vessels: Karlsruher Flaschen holding 10 neonates in 250-300 mL of test medium
- Experimental design: 4 test concentrations plus 1 control; 10 neonates per vessel, 2 replicates per concentration/control; no feeding during the exposure period; closed bottle conditions
- Method of initiation: neonates were placed in prepared media
- Photoperiod: 16 h light: 8 h dark
- Temperature: mean +/- 1 °C (in the temperature range 18 - 22 °C)
- Aeration: none
- Method of administration: stock solution
- Medium renewal: none
- Criteria of effects: The criterion of adverse effects used in this study was the substance-induced alteration of the normal mobility behaviour and the loss of locomotory actions of the neonates, observed at 24 and 48 hours
Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.31 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
0.62 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
0.155 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
The results are expressed in terms of mean measured concentrations (at 48h). Measured concentrations ranged from 69.6 - 86% of nominal values at 0 hours, and from 45.6- 52% of nominal values at 48 hours, respectively.
Validity criteria fulfilled:
yes
Conclusions:
After 48 hours of exposure both an EC0 of 0.155 mg/L and an LC100 of 0.62 mg/L was measured. The geometric mean of the two values corresponding to the EC 50 value is 0.31 mg/L. The results are expressed in terms mean measured concentrations (at 48h). Measured concentrations ranged from 69.6 - 86% of nominal values at 0 hours, and from 45.6- 52% of nominal values at 48 hours, respectively.
Executive summary:

A study was performed to assess the acute toxicity to Daphnia magna STRAUS under static conditions in a closed bottle system. The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC (O.J. No. L383A, 29.12.92) Part C, Method 2 'Acute toxicity for Daphnia' which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 202 'Daphnia sp., Acute Immobilisation Test and Reproduction Test, Part I - The 24h EC50 Acute Immobilisation Test'. The Daphnia were exposed to a range of concentrations, nominally 0.125, 0.25, 0.5 and 1 mg/L of test item dissolved in water. After 48 hours of exposure both an EC0 of 0.155 mg/L and an LC100 of 0.62 mg/L was measured. The geometric mean of the two values corresponding to the EC50 value is 0.31 mg/L. The results are expressed in terms of mean measured concentrations (at 48h). Measured concentrations ranged from 69.6 - 86% of nominal values at 0 hours, and from 45.6- 52% of nominal values at 48 hours, respectively. The hardness of the dilution water used was 15.0 °dH. This toxicity study is classified as acceptable and satisfies the guideline requirements for the acute daphnia study.

Description of key information

A study was performed to assess the acute toxicity to Daphnia magna STRAUS under static conditions in a closed bottle system. The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC (O.J. No. L383A, 29.12.92) Part C, Method 2 'Acute toxicity for Daphnia' which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 202 'Daphnia sp., Acute Immobilisation Test and Reproduction Test, Part I - The 24h EC50 Acute Immobilisation Test'. The Daphnia were exposed to a range of concentrations, nominally 0.125, 0.25, 0.5 and 1 mg/L of test item dissolved in water. After 48 hours of exposure both an EC0 of 0.155 mg/L and an LC100 of 0.62 mg/L was measured. The geometric mean of the two values corresponding to the EC50 value is 0.31 mg/L. The results are expressed in terms of mean measured concentrations (at 48h). Measured concentrations ranged from 69.6 - 86% of nominal values at 0 hours, and from45.6- 52% of nominal values at 48 hours, respectively. The hardness of the dilution water used was 15.0 °dH.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
0.31 mg/L

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