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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Deviations:
no
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not applicable
GLP compliance:
yes
Analytical monitoring:
yes
Remarks:
Gas Chromatography (GC)
Details on sampling:
Sampling schedule of chemical analysis:
- Control: on Nov. 19, Nov. 20, Nov. 21 and Nov. 22, 2001 in the freshly prepared medium; on Nov. 20, Nov. 21, Nov. 22 and Nov. 23 after 24 h of exposure
- Test concentrations: on Nov. 19, Nov. 20, Nov. 21 and Nov. 22, 2001 in the freshly prepared medium; on Nov. 20, Nov. 21, Nov. 22 and Nov. 23 after 24 h of exposure
Vehicle:
no
Details on test solutions:
To produce the highest test concentration (1 mg/L) the test substance was added daily to 5 litre of dilution water, then treated with an ultra turrax for 60 seconds and afterwards stirred for 24 hours on a magnetic stirrer. Finally undissolved particles of the test substance were removed by filtration. The test concentrations 0.125, 0.25, and 0.5 mg/L were prepared daily by diluting a stock solution (0.5 mg/L) and treated in the same way as the test concentration 1 mg/L.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
- Source: Bio International B. V. (Netherlands)
- Date of birth: April 6, 2001
- Date supplied: Sept. 5, 2001
- Acclimatisation: Stock held since Sept. 5, 2001 and acclimatised to the test conditions since then
- Temperature: 20 - 24 °C
- Dissolved oxygen: > 5 mg/I
- Feeding: Commercial fish food, daily (Discontinued 24 h prior to test
- Mortalities during acclimatisation period: <2 %
- Medication: None
- Mean standard length (n = 50): 3.2 cm (S.D. = 2.7 cm)
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
223.1 mg CaCO3/L
Test temperature:
21.4 °C (concerning the highest test concentration)
pH:
7.7 (concerning the highest test concentration)
Dissolved oxygen:
8.6 mg/L (concerning the highest test concentration)
Salinity:
n.a.
Conductivity:
No data
Nominal and measured concentrations:
- Test concentrations (nominally): 0.125, 0.25, 0.5 and 1 mg/L
- Test concentrations (measured): in the range between 62.4 and 92% of nominal values in the freshly prepared medium and between 20 and 52% of nominal values after 24 hours of exposure (semi-static)
Details on test conditions:
Exposure conditions:
- Test vessels: glass aquaria holding 5 L of test media
- Experimental design: 4 test concentrations plus 1 control; 10 animals per test concentration; no feeding during the exposure period
- Method of initiation: animals were placed in prepared media
- Loading: 0.61 g body weight (wet weight) per litre
- Photoperiod: 16 h light: 8 h dark
- Temperature: mean +/- 1 °C (in the temperature range 20 - 24 °C)
- Aeration: gentle aeration via narrow glass tubes
- Method of administration: direct weighing; stock solution
- Medium renewal: yes
- Criteria of effects: The criterion of death used in this study was the absence of response to physical stimulation. In addition to observations on mortality at 2, 24, 48, 72 and 96 hours, subjective assessments were made on the abnormal behaviour and swimming action
Reference substance (positive control):
no
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
0.13 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
0.3 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
0.053 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
All results are expressed in terms of mean measured concentrations. Measured concentrations ranged from 62.4 - 92% of nominal values in the freshly prepared medium and from 20-52% of nominal values after 24 hours of exposure (semi-static)
Sublethal observations / clinical signs:

One test fish had a somewhat larger size than the one recommended in the test guideline for the zebra fish. This deviatiation is not regarded to be relevant to the results. The temperature fluctuation in the course of the study exceeds 1 °C which is not regarded to be relevant to the results.

Conclusions:
After 96 hours of exposure an LC0 of 0.053 mg/L and an LC100 of 0.3 mg/Lwas measured for the test substance towards fish. The geometric mean of the two values corresponding to the LC50 value is 0.13 mg/L. All results are expressed in terms of mean measured concentrations. Measured concentrations ranged from 62.4 - 92% of nominal values in the freshly prepared medium and from 20 - 52% of nominal values after 24 hours of exposure (semi-static test setup)
Executive summary:

The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC (O.J. No. L383A, 29.12.92) Part C, Method 1 'Acute toxicity for Fish' which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test'. Groups of ten fish of the recommended size were exposed under semi-static conditions to a range of concentrations, nominally 0.125, 0.25, 0.5 and 1 mg/L of test item dissolved in water. Auxiliaries used to prepare the ·test media were an ultrasonic bath, a magnetic stirrer and folded filters. Observations were made on the numbers of dead fish and the incidence of sub-lethal effects after 2, 24, 48, 72 and 96 hours of exposure. After 96 hours of exposure both an LC 0 of 0.053 mg/L and an LC 100 of 0.3 mg/L was measured. The geometric mean of the two values corresponding to the LC50 value is 0.13 mg/L. All results are expressed in terms of mean measured concentrations. Measured concentrations ranged from 62.4 - 92% of nominal values in the freshly prepared medium and from 20 - 52% of nominal values after 24 hours of exposure.

Description of key information

The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC (O.J. No. L383A, 29.12.92) Part C, Method 1 'Acute toxicity for Fish' which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test'. Groups of ten fish of the recommended size were exposed under semi-static conditions to a range of concentrations, nominally 0.125, 0.25, 0.5 and 1 mg/L of test item dissolved in water. Auxiliaries used to prepare the ·test media were an ultrasonic bath, a magnetic stirrer and folded filters.Observations were made on the numbers of dead fish and the incidence of sub-lethal effects after 2, 24, 48, 72 and 96 hours of exposure.After 96 hours of exposure both an LC 0 of 0.053 mg/L and an LC 100 of 0.3 mg/L was measured. The geometric mean of the two values corresponding to the LC50 value is 0.13 mg/L. All results are expressed in terms of mean measured concentrations. Measured concentrations ranged from 62.4 - 92% of nominal values in the freshly prepared medium and from 20 - 52% of nominal values after 24 hours of exposure.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
0.13 mg/L

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