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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
basic toxicokinetics in vitro / ex vivo
Adequacy of study:
other information

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
The metabolism of dibenzylamine: identification of N,N-dibenzylhydroxylamine as the major in vitro metabolic procuct from rabbit fortified hepatic homogenates.
Author:
Beckett AH, Coutts RT, Gibson GG
Year:
1975
Bibliographic source:
J Pharm Pharmacol 7727, 659-665
Reference Type:
publication
Title:
Microsomal N-hydroxylation of dibenzylamine
Author:
Beckett AH, Gibson GG
Year:
1975
Bibliographic source:
Xenobiotical 5, 677-686

Materials and methods

Objective of study:
metabolism
Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Homogenized male rabbit liver 9000g supernatant was incubated with N,N-Dbenzylamine under aerobic conditions for 60 minutes.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Dibenzylamine
EC Number:
203-117-7
EC Name:
Dibenzylamine
Cas Number:
103-49-1
Molecular formula:
C14H15N
IUPAC Name:
dibenzylamine
Details on test material:
purity: laboratory reagent grade ( no further detail)
Radiolabelling:
no

Test animals

Species:
other: rabbit liver
Strain:
New Zealand White
Sex:
male

Administration / exposure

Route of administration:
other: isolated rat liver 9000 g supenatant was treated
Vehicle:
water
Duration and frequency of treatment / exposure:
once 60 min
Doses / concentrations
Remarks:
Doses / Concentrations:
10 µmol
No. of animals per sex per dose / concentration:
in vitro investigation
Control animals:
other: control incubations (1) rabbit liver homogenate without substate

Results and discussion

Main ADME resultsopen allclose all
Type:
metabolism
Results:
The study has shown that N-oxidation is the major metabolic process. Of the total amount of dibenzylamine metabolized , 90 % is converted to N,N-dibenzylhydroxylamine. The primary amine benzylamine is formed to 6 % of total substrate metabolized.
Type:
metabolism
Results:
The N-oxidation of the parent amine was inhibited by CO, SKF525-A, and inhibitors known to interact with microsomal cytochrom P-450. Phenobarbitone pre-treatment stimulates further metabolism of the hydroxylamine.

Toxicokinetic / pharmacokinetic studies

Details on absorption:
no data, in vitro investigation
Details on distribution in tissues:
no data, in vitro investigation
Details on excretion:
no data, in vitro investigation

Metabolite characterisation studies

Metabolites identified:
yes
Details on metabolites:
main metabolite: N,N-dibenzylhydroxylamine, identity established by thin-layer-chromatography, , gas-liquid chromatography and combined gas-liquid chromatography
minor metabolite: benzylamine

Applicant's summary and conclusion

Executive summary:

N,N-Dibenzylamine metabolism was studied in vitro by incubation of N,N-dibenzylamine with male rabbit liver 9000g supernatant for 30 minutes. The study has shown that N-oxidation is the major metabolic process. Of the total amount of dibenzylamine metabolized , 90 % is converted to N,N-dibenzylhydroxylamine. The primary amine benzylamine is formed to 6 % of total substrate metabolized. The N-oxidation of the parent amine was inhibited by CO, SKF525-A, and inhibitors known to interact with microsomal cytochrom P-450. Phenobarbitone pre-treatment stimulates further metabolism of the hydroxylamine (Beckett 1975).