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Environmental fate & pathways

Biodegradation in water: screening tests

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Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1995-07-11 to 1995-08-08
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
Deviations:
no
Principles of method if other than guideline:
The substance is einwogen in a mineral medium inoculated with a mixed population of aquatic microorganisms and 28 days under aerobic conditions and incubated in the dark at 20 ± 1 °C. The biodegradability of the substance is determined during this time using a respirometer, which records the oxygen consumption.
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- species: mixed population of aquatic microorganisms (activated sludge)
- source: Laboratory activated sludge plant is operated with municipal wastewater (Wupperverband)
- day of collection: 1995-07-10
- no pretreatment
- concentration of inoculum: 30 mg/L
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Reference substance:
aniline
Remarks:
at least 99.5 %
Key result
Parameter:
% degradation (O2 consumption)
Value:
3
Sampling time:
28 d
Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
Whitin 28 days, a degradation of 3 % was determined for dibenzylamine and hence dibenzylamine is considered to be "Not Readily Biodegradable".
Executive summary:

A 28-d ready biodegradability test (Manometric respirometry test) indicated that dibenzylamine was not readily biodegradable under the conditions of the test. The test substance showed only 3 % biodegradation. Dibenzylamine is considered to be "Not Readily Biodegradable".

Endpoint:
biodegradation in water: inherent biodegradability
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Reliable source
Qualifier:
according to guideline
Guideline:
OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
Deviations:
no
Principles of method if other than guideline:
The principle of the method is to ventilate the test substance in mineral nutrient solution with activated sludge under stirring. The test is the only inflicted carbon source. the degradation process by regular determination of the dissolved carbon content (DOC) or chemical oxygen (COD) monitors and described in a degradation curve.
GLP compliance:
not specified
Oxygen conditions:
aerobic
Inoculum or test system:
sewage, domestic (adaptation not specified)
Details on inoculum:
- source: biological treatment plant HOECHST AG
- activaed sludge dry substance 1.1 ± 0.1 g/L
- DOC concentration of 50 - 400 mg/L
- COD concentration of 200 - 1000 mg/L
Duration of test (contact time):
34 d
Key result
Value:
100
Sampling time:
34 d
Interpretation of results:
inherently biodegradable

Description of key information

Two studies (Wellens, 1990 and Bayer, 1995) were included in this dossier and regarded as key studies (Klimisch score of 2). The biodegradability of dibenzylamine was determined according to OECD Guideline 302B (Wellens, 1990) and EU Method C.4 -D (Bayer, 1995). Under the conditions of the test, dibenzylamine was determined to be not biodegradable within 28 days.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed
Type of water:
freshwater

Additional information