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Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
from 1990-10-01 to 1990-10-05
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions
Remarks:
GLP study, no OECD guideline but national guideline followed, stability, homogenity and concentration of the test item were not investigated.
Qualifier:
according to guideline
Guideline:
ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
Version / remarks:
1986 (E)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
activated sludge
Details on inoculum:
- Source: Activated sludge of laboratory STP (OECD), non-adapted
- Sampling date: 28.09.1990
- Pretreatment: no
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Nominal and measured concentrations:
1000, 1800, 3200, 5600, 10000 mg/L (nominal concentrations).
No measured values.
Details on test conditions:
- TEST CONCENTRATIONS
- Concentration of inoculum: 6 g/L dry substance
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol
Key result
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
1 770 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: confidence interval: 1230 - 2290 mg/L
Key result
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
350 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: confidence interval: 122 - 608 mg/L
Details on results:
Test concentration (mg/L) / respiration rate (mg/Lh) / inhibition (%):
- 1000 / 25.5 / 32.0
- 1800 / 19.2 / 48.8
- 3200 / 11.0 / 70.7
- 5600 / 6.0 / 84.0
- 10000 / 4.0 / 89.3


EC values and confidence intervals:
- EC05: 221 mg/L (61 - 427 mg/L)
- EC10: 350 mg/L (122 - 608 mg/L)
- EC50: 1770 mg/L (1230 - 2290 mg/L)
- EC90: 8950 mg/L (6180 - 17300 mg/L)
- EC95: 14200 mg/L (8900 - 33700 mg/L)
Validity criteria fulfilled:
yes
Conclusions:
The 3 h-EC10 and 3 h-EC50 values were determined to be 350 and 1770 mg/L, respectively.
Executive summary:

The purpose of the 3-hour test was to evaluate the influence of the test item on the activity of activated sludge by measuring the respiration rate. Following concentrations were tested 1000, 1800, 3200, 5600 and 10000 mg/L. The 3 h-EC10 and 3 h-EC50 values were calculated to be 350 and 1770 mg/L, respectively.

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
from 1988-07-18 to 1988-07-18
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
no
Qualifier:
according to guideline
Guideline:
ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
Version / remarks:
1986
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
activated sludge
Details on inoculum:
- non adapted activated sludge
- Source: laboratory STP
- Date of extraction: 24.06.1988
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Test temperature:
20.9 - 23.8 °C
21.4 - 23.6 °C
pH:
7.4 - 8.0
7.7 - 7.8
Nominal and measured concentrations:
Nominal concentration: 100, 1000 and 10000 mg/L; 1000, 1800, 3200, 5600, 10000 mg/L.
No measured concentrations.
Details on test conditions:
TEST SYSTEM
- Test vessel: 3L equipement

TEST MEDIUM
- synthetic culture medium
- ingredients: pepton, meat extract, urea, natrium chloride, calcium chloride dihydrate, magnesium sulfate heptahydrate, dipotassium phosphate

TEST CONCENTRATIONS
- concentration of activated sludge: 6.00 g/L
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol
Key result
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
894.07 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: confidence interval: 206.51 - 1544.57 mg/L
Key result
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
3 534.04 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: confidence intercal: 2343.43 - 5309.6 mg/L
Details on results:
1st test:
Test item concentration (mg/L) / respiration rate (mg/Lh) / inhibition (%):
- 100 / 19 / 0
- 1000 / 17 / 8
- 10000 / 6 / 67

2nd test:
Test item concentration (mg/L) / respiration rate (mg/Lh) / inhibition (%):
- 1000 / 15 / 0
- 1800 / 10 / 33
- 3200 / 6 / 60
- 5600 / 6 / 60
- 10000 / 3 / 80

The respiration rate of the control was 15 mg/Lh.

EC values and confidence intervals:
- EC05: 605.48 mg/L (96.46 - 1169.89 mg/L)
- EC10: 894.07 mg/L (206.51 - 1544.57 mg/L)
- EC50: 3534.04 mg/L (2343.43 - 5309.59 mg/L)
- EC90: 13971.52 mg/L (8112.19 - 59860.58 mg/L)
- EC95: 20630.79 mg/L (10712.71 - 128119.05 mg/L)

Other:
Due to its physical-chemical properties a part of the test item was accumulated on the pH electrode. Therefore, the nominal concentrations do not exactly match the real concentrations in the test vessels.
Results with reference substance (positive control):
1st test:
Concentration of reference substance (mg/L) / respiration rate (mg/Lh) / inhibition (%):
- 1 / 16 / 10
- 20 / 6 / 67

2nd test:
Concentration of reference substance (mg/L) / respiration rate (mg/Lh) / inhibition (%):
- 1 / 14 / 9
- 20 / 6 / 60

Validity of test test was confirmed by graphical analysis using the reference substance.

Validity criteria fulfilled:
yes
Conclusions:
The 3 h-EC10 and 3 h-EC50 values were determined to be 894 and 3535 mg/L, respectively.
Executive summary:

The purpose of the 3-hour test was to evaluate the influence of the test item on the activity of activated sludge by measuring the respiration rate in accordance with OECD guideline 209. Following concentrations were tested 1000, 1800, 3200, 5600 and 10000 mg/L. The 3 h-EC10 and 3 h-EC50 values were calculated to be 894.07 and 3534.04 mg/L, respectively.

Description of key information

The 3 h-EC10 and 3 h-EC50 values were determined to be 350 and 1770 mg/L, respectively.

Key value for chemical safety assessment

EC50 for microorganisms:
1 770 mg/L
EC10 or NOEC for microorganisms:
350 mg/L

Additional information

Key study:

The purpose of the 3-hour test was to evaluate the influence of the test item on the activity of activated sludge by measuring the respiration rate. Following concentrations were tested 1000, 1800, 3200, 5600 and 10000 mg/L.

The 3 h-EC10 and 3 h-EC50 values were calculated to be 350 and 1770 mg/L.

Supporting study:

The purpose of the 3-hour test was to evaluate the influence of the test item on the activity of activated sludge by measuring the respiration rate in accordance with OECD guideline 209. Following concentrations were tested 1000, 1800, 3200, 5600 and 10000 mg/L. The 3 h-EC10 and 3 h-EC50 values were calculated to be 894.07 and 3534.04 mg/L.