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EC number: 925-259-5
CAS number: 140921-24-0
The toxicity to algae was assessed according to EU method C.3. In this 72-hour toxicity test with freshwater algal species Desmodesmus subspicatus, the EbC50 and ErC50 values were determined to be 29 mg/L and 43 mg/L (nominal concentrations, WAF), respectively. The no-observed-effect concentration (NOEC) and lowest-oberved-effect-concentration was determined to be 12.5 mg/L and 25 mg/L (both nominal concentrations, WAF), respectively.
A study was performed to assess adverse
effects of the test item on the growth (= increase in cell density) and
the growth rate (=rate of increase of cell density with time) of the
planktonic freshwater algal species Desmodesmus subspicatus over several
generations. The study was conducted in accordance with EEC methods for
Determination of Ecotoxicity Annex to directive 92/69/EEC Part c, Method
3 which is in most parts equivalent o the OECD guideline for testing of
chemicals no. 201.
Exponentially growing algal cells were
exposed for a period of 72 h to a range of concentrations, nominally
6.25, 12.5, 25, 50 and 100 mg/L of the test item dissolved in water.
Auxiliaries used to prepare the test media were an ultra turrax, a
magnetic stirrer and folded flters. The cell densities were measured at
24 h intervals. Inhibition of the algal population was measured as
reduction in growth (index b) and growth rate (index r) relative to
control cultures grown under identical conditions. Growth and growth
rates were used to calculate a No Observed Effect Concentration and a
Lowest Observed Effect Concentration according to Dunnett (1994, 1995).
The following values were determined: EbC50 (72 h)=29 mg/L; ErC50 (72
h)=43 mg/L; NOEC (b)=12.5mg/L; LOEC (b)= 25 mg/L; NOEC (r)= 12.5 mg/L
and LOEC (r)=25 mg/L. The results were expressed in terms of effective
loadings. Considering the heterogeneous composition of the test item,
Water Acomodated Fractions (WAF) were tested. The results of DOC
determinations reflect a rough impression only of exposure conditions
over time. Not informations are available on a molecular weight of the
test item and on a potential correlation between effective loadings and
measured DOC values.
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