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EC number: 238-405-1
CAS number: 14433-76-2
In a study according to OECD test guideline 216 under GLP, the toxicity of the test substance to soil microorganisms was determined to be 0.53 g/kg soil dw (EC10), 0.87 g/kg soil dw (EC20) and 2.29 g/kg soil dw (EC50), respectively.
The toxicity of the test substance to soil microorganisms was
investigated in a study according OECD test guideline 216 under GLP.
A freshly collected and sieved sandy loam field soil (standard
soil Lufa 2.3) was used for the test. The soil was amended with finely
ground lucerne grass green meal (5.0 g kg-1soil dry mass).
Five test item concentrations were tested: 0.10 g, 0.316 g, 1.00 g, 3.16
g and 10.0 g kg-1soil dry mass. Respective amounts of
the test item were diluted in water and mixed into portions of the soil.
Each treatment was set up withthreereplicates,
each in appropriate glass vessels and incubated for a test period of 28
days at 20 ± 2°C in the dark.
Variability in the
control revealed a maximum deviation from the mean nitrate concentration
of 2.4%. Therefore, the validity criterion of a variability of less than
15% within the control given by the guideline was fulfilled. Nitrate
extracted from soils treated with test item at 0.1 g/kg soil and 0.32
g/kg soil exceeded the nitrate concentration in extracts from the
untreated controls by 20% and 61%, respectively. In view of the inherent
variability of microbiological test systems, the ecological relevance of
this observation remains doubtful.
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