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Diss Factsheets
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EC number: 209-218-2 | CAS number: 561-41-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: OECD 410
- Deviations:
- yes
- Remarks:
- results extracted from sub-acute (28 day) study
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- Ferrate(4-), hexakis(cyano-C)-, methylated 4-[(4-aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]benzenamine copper(2+) salts
- EC Number:
- 235-468-7
- EC Name:
- Ferrate(4-), hexakis(cyano-C)-, methylated 4-[(4-aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]benzenamine copper(2+) salts
- Cas Number:
- 12237-62-6
- Molecular formula:
- [C24N3H28]CuFe(CN)6
- IUPAC Name:
- Ferrate(4-), hexakis(cyano-C)-, methylated 4-[(4-aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]benzenamine copper(2+) salts
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Fifty healthy wistar albino rats of both sex, body weight ranging 200±20 gm selected for study. All the selected animals acclimatized for standard laboratory condition for period of one week. After acclimatization animal were divided into five groups (one control and four treated) each having five male and five female. All the animals were prepared 24 hrs prior to application of test compound. The test substance applied uniformly 62.5, 250 and 1000 mg/kg b.wt for at least 6 hours per day on a 7-day per week basis. A reversal group was also maintained in same manner at the highest test dose level 1000 mg/kg b.wt for period of 42 days. The signs of toxicity were recorded as they are observed, including the time of onset, the degree and duration. Cage-side observations viz; changes in skin and fur, eyes and mucous membranes and also respiratory, circulatory, autonomic and central nervous system, somatomotor activity and behaviour pattern. All the animals were sacrificed on day 29th of experiment for necropsy examination and histopathological examination of organ and tissue collection was done after completion of study.
Environmental conditions: The animals were kept in air conditioned rooms with 10-15 air circulation cycle per hour, temperature between 22-250C, relative humidity 40-60% and illumination cycle set to 12 hours artificial fluorescent light and 12 hours dark.
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- water
- Details on dermal exposure:
- Dose preparation of pigment violet 27 was done freshly, few minutes prior to dosing. The test substance Pigment Violet-27 was mixed with distilled water to obtain paste. The test substance was applied uniformly daily over an area approximately 10 per cent of the total body surface area in graduated doses for a period ranging from 1-28 days. Between applications the test substance is held in contact with the skin with a porous gauze dressing and non-irritating tape. The test site was covered in a suitable manner to retain the gauze dressing and test substance and ensure that the animals cannot ingest the test substance.
- Duration of exposure:
- 7 days
- Doses:
- 62.5 mg/L, 250, 1000 mg/L
- No. of animals per sex per dose:
- 5
- Control animals:
- yes
- Details on study design:
- Group Dose (mg/kg) Treatment 28-Days
Male Female
Group-I Vehicle control 5 5
Group-II 62.5 mg/kg b. wt 5 5
Group-III 250 mg/kg b. wt 5 5
Group-IV 1000 mg/kg b. wt 5 5
Group-V 1000 mg/kg b. wt 5 5
Total nos of animal 25 male & 25 female - Statistics:
- The data obtained from present investigation were analyzed by One Way ANOVA and Tukey HSD Test.
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- other: NOAEL
- Effect level:
- > 1 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- No mortality was observed
- Clinical signs:
- other: The treated animals were closely observed for clinical signs of intoxication, first 4 hours and every 1 hrs interval for 24 hrs after dosing and thereafter twice a day for 28 days. All the rats were observed at least twice daily to observe any clinical si
- Gross pathology:
- During necropsy all the organs viz; Adrenals, Brain, Heart, Kidneys, Liver, Lungs, intestine, Spleen, lymph nodes and Testes of body were examined for gross pathological changes.
Any other information on results incl. tables
There was no dermal irritation observed in the exposed rats at the end of 7 days
Applicant's summary and conclusion
- Interpretation of results:
- practically nontoxic
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- There was no mortality and no clinical signs of dermal irritation observed in the rats after 7 days of continuous exposure to pigment violet 27 in dose ranging from 62.5 mg/kg bw upto 1000 mg/kg bw. Thus, it can be cioncluded that pigment violet 27 is not acutely toxic to Wistar rat by the dermal route
- Executive summary:
There was no mortality and no clinical signs of dermal irritation observed in the rats after 7 days of continuous exposure to pigment violet 27 in dose ranging from 62.5 mg/kg bw upto 1000 mg/kg bw. Thus, it can be cioncluded that pigment violet 27 is not acutely toxic to Wistar rat by the dermal route
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