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EC number: 247-148-4
CAS number: 25637-99-4
Table 4. Mean
concentration and standard deviation (SD) of nitrate in soil treated wit
the test item and in non treated soil (control and solvent control) on
day 0 and day 28 of the test.
minimum and maximum nitrate concentration, and maximum deviation from
the mean in the non treated soil (control) at the start (day 0) and at
the end (day 28) of the test.
validity criterion concerning the variation between replicate control
samples in the Nitrogen Transformation Test (concentration of nitrate)
was fulfilled: the maximum deviation was 10.07%, and was therefore
within the acceptable range of deviation of <15% (Table 2).
(2007) Hexabromocyclododecane (HBCD): Effects on Soil Microorganisms.
ECT Oekotoxikologie GmbH, D-65439 Flörsheim, Germany, unpublished report
number AU1BB, 20 pages plus an Appendix of 37 pages (Study Plan 11
pages; Analytical Letter Report 26 pages).
Guideline for the Testing of Chemicals No. 216 “Soil Microorganisms:
Nitrogen Transformation Test”.
from the Guidelines:
of quartz sand added to the soil was 4% instead of 1%.
(HBCD); CAS RN: 3194-55-6; Batch No.: 6541; Content (a.i.): 99.6%.
amounts of the test item were diluted in acetone and mixed into quartz sand.
After evaporation of the acetone the sand was mixed into sieved field
soil (Lufa standard soil
2.3) that was amended with ground lucerne meal (5.0 g kg-1soil)
and adjusted to a watercontent
of 50% of the maximum water-holding capacity. The nominal test item
10 mg kg-1, 31.6 mg kg-1, 100 mg kg-1,
316.2 mg kg-1, and 1000 mg kg-1soil dry weight.
There were three replicates per test item concentration, and three per
control and solvent control,
each. The soil was incubated in glass jars for a test period of 28 days
at 20 ± 2 °C in the dark.
Soil nitrate concentrations were measured on day 0 (i.e. within 24-h
after test start), and on
day 28 of the test (28 days after test item application).
nitrate concentrations on day 28 were in the range of 54.1 mg kg-1 to
62.3 mg kg-1 soil dry mass in the control and treated soil
variation of the nitrate concentrations within the three control
specimens was less than ± 15% of the mean. Therefore, the validity
criteria defined in the guideline have been fulfilled.
addition of HBCD ranging from 10 to 1000 mg/kg. d.w. soil (nominal
concentrations) did notlead
to any significant effect (ANOVA at p≤0.05)
on the formation of nitrate in the treated soil samples as compared to
the control soils.
can be concluded that the EC50
HBCD is >1000 mg/kg dry soil, whereas the NOEC is considered to be 1000
mg/kg dry soil.
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