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EC number: 247-148-4
CAS number: 25637-99-4
Ear Swelling (Irritation, range finding test)
Percent Ear Swelling
(n=l/group) were treated topically with vehicle or HBCD preparation for
two consecutive days. Ear thickness was measured using a digital
micrometer. Values represent mean for each mouse (n=2 ears/dose).
Local Lymph Node Assay (LLNA) assesses the potential of test materials
to cause contact sensitization by measuring the lymphocyte proliferative
responses from auricular lymph nodes following topical application of
the test materials to mouse ears. Test materials that elicit a
Stimulation Index (SI) of >3 (i.e., 3-fold greater proliferation than
control animals) should be considered positive for dermal sensitization
mice received one of three concentrations of HBCD (2%, 20% or 50% w/v)
or DMF (dimethylformamide) on days 1-3 (n=6 mice/group). HCA (a-hexyl
cinnamaldehyde), a moderate contact sensitizer, was evaluated
concurrently as a positive dermal sensitization control. The test
materials were administered to the dorsal surface of both ears (25
µl/ear). On day 6, all mice received an intravenous tail vein injection
of phosphate buffered saline containing 20 µCi of 3H-thymidine.
Uptake into the auricular lymph nodes draining the site of chemical
application was measured 5 hours later.
weight data were unremarkable and minor increases in ear thickness were
noted suggesting slight irritation following applications of 20% and 50%
HBCD. There were no indications that HBCD possesses dermal sensitization
potential. SI values were consistently around 1.0 at all doses tested.
Lymphocyte proliferation by DMF, vehicle treated mice (2015 dpm) was
higher than historical laboratory values commonly observed using acetone
and olive oil as a vehicle. This is not inconsistent with that reported
in the literature for this vehicle. HCA administrations (30% v/v)
elicited proliferation that was 3-fold greater than that of vehicle
controls thus detecting the moderate contact sensitization potential in
this study. On the basis of these results, HBCD would not be considered
to have contact sensitization potential. HBCD therefore does not meet
the criteria for classification as a sensitiser as set out by 67/458/EEC.
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