6-[C12-18-alkyl-(branched, unsaturated)-2,5-dioxopyrrolidin-1-yl]hexanoic acid

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991-12-12

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: non-GLP Guideline study, limited reporting

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

6-[3-(C12-C18)-alkenyl(branched, unsaturated)-2,5-dioxopyrrolidin-1-yl]hexanoic acid

EC Number:

701-162-1

Molecular formula:

C22H37NO4 - C28H49NO4

IUPAC Name:

6-[3-(C12-C18)-alkenyl(branched, unsaturated)-2,5-dioxopyrrolidin-1-yl]hexanoic acid

Details on test material:

Lot No. 3/90 (24.04.1990)

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

500-1000 mg/L

Test organisms

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

Kommunale Abwasserreinigungsanlage Frankfurt-Sindlingen, non adapted sludge

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Post exposure observation period:

none

Test conditions

Hardness:

n.a.

Test temperature:

20 +/- 2 deg C

pH:

7.2 +/- 0.2

Dissolved oxygen:

saturated

Reference substance (positive control):

yes

Remarks:

3,5 Dichlorophenol 5-20-30 mg/L

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

3 h

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The registration substance did not inhibit the respiration rate of microorganisms in activated sludge at the highest tested concentration of 1000 mg/L in an OECD 209 test. The NOEC (3h) is 1000 mg/L.