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EC number: 701-162-1
CAS number: -
inherent biodegradability/elimination of the test item
was determined in the Zahn-Wellens-Test / EMPA Test with a non adapted
activated sludge over a period of 28 days. The definitive study was
conducted from 2012-08 -20 to 2012-09-19
according to OECD guideline 302 B at Dr.U.Noack-Laboratorien,D-31157
The test item was tested at a concentration of 40
mg/L in duplicates, corresponding to a DOC of 28.3
mgC/L in the test vessel. The biodegradation/elimination of the test
item was followed by determination of DOC in filtered samples. The ratio
DOC to activated sludge dry weight at the beginning was 1:7. The ratio
of eliminated DOC, corrected for the inoculum control to the initial DOC
value is expressed as the percentage biodegradation at each sampling
order to check the activity of the test system diethylene glycole at a
concentration of 120 mg/L was used as functional control. After 7 days a
biodegradation rate of 100 % was reached.
physico-chemical elimination of the test item was checked in the sterile
control (without inoculum and poisoned with HgCl2) at the
test item concentration of 40
mg/L. At test start only 67 % of the nominal concentration was
determined in the test solution. Until day 7 the concentration increased
to 77 % of the nominal concentration and from day 14 until test end the
concentration was ≥
of the nominal concentration. It is assumed that the lower concentration
until day 7 is due to incomplete dissolution of the test item in the
test solution. The increase of the test concentration indicated that the
test item was completely dissolved after 14 days. No physico-chemical
elimination occurred in the sterile control after 28 days.
biodegradation/elimination reached the 10 % level after 3 days. The
biodegradation/elimination remained in the range of 22 -35 % and the
level of 70 % was not reached within the test duration of 28 days. After
28 days a biodegradation of 22 % was determined.
to the criteria of the OECD 302B guideline the test item is
not inherently, ultimately biodegradable within 28 days. But the
results indicate that the test item is inherently, primary
Biodegradability/Elimination of the Test Item
(Pentapropylensuccinimido)-capronic acid in
Comparison to the Functional Control and Sterile
Inherent Biodegradation / Elimination [%]
Study Day [d]
# assumed due to incomplete dissolution of
the test item
* physico-chemical elimination
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