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EC number: 701-162-1
CAS number: -
Rates in the Definitive Test after 24 and 48 h of Exposure
= 20, divided into 4 replicates with 5 daphnids each)
Test Item Concentration
In the acute immobilisation test with Daphnia
magna (STRAUS), the effects of the test item (Pentapropylensuccinimido)-capronic
acid (batch number: ESD0009272) were determined according to OECD 202
(2004) from 2013-01-28 to 2013-02-01, with the definitive exposure phase
from 2013-01-29 to 2013-01-31, at Dr.U.Noack-Laboratorien,
Käthe-Paulus-Str.1, 31157 Sarstedt, Germany.
The study was conducted under static
conditions over a period of 48 h. Six concentration levels of the test
item (Pentapropylensuccinimido)-capronic acid in the range of 2.50 to
80.0 mg/L were prepared with dilution water in a geometric series with a
separation factor of 2 and tested. Twenty daphnids were exposed to each
concentration level and the control.
The concentrations of the test item (Pentapropylensuccinimido)-capronic
acid were analytically
verified by LC-MS at the start of the exposure (0 h) and at the end of
the exposure (48 h) of all concentration levels and the control. Details
of the analytical method are presented in part 11.
The measured concentrations of the
test item at the start of the exposure (0 h) were in the range of 83 to
102 % of the nominal values. The measured concentrations of the test
item at the end of the exposure (48 h) were in the range of 44 to 49 %
of the nominal values. The geometric mean measured concentrations were
calculated to be: 1.57 – 3.13 – 6.23 – 12.6 – 25.1 – 56.8 mg/L. The
analytical results are presented in Table
endpoints were assessed based on the geometric mean measured
concentrations of the test item (Pentapropylensuccinimido)-capronic acid
quality parameters (i.e. pH-values and dissolved oxygen concentrations),
measured at the start (0 h) and the end of the exposure (48 h), were
within the acceptable limits. The validity criteria of the test
guideline were fulfilled.
(with Confidence Interval), EC100-Values
on the geometric mean measured concentrations of the test item)
Geometric Mean Measured
Concentrations of the Test Item
95 % confidence limits
7.31 – 18.6
1.57 – 2.80
12.4 – 21.6
1.85 – 2.97
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