6-[C12-18-alkyl-(branched, unsaturated)-2,5-dioxopyrrolidin-1-yl]hexanoic acid

Bioaccumulation: aquatic / sediment

Administrative data

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

1995-07-25 to 1995-10-24

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP study according a Japanese Guideline (no details given), well and detailed reported, deficiencies in translation from Japanese

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Japanese METI Guideline according Test methods for New Chemical Compounds

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

6-(3-tetrapropenyl-2,5-dioxopyrrolidin-1-yl)hexanoic acid

EC Number:

800-770-5

Cas Number:

1424148-99-1

Molecular formula:

C22H39NO4

IUPAC Name:

6-(3-tetrapropenyl-2,5-dioxopyrrolidin-1-yl)hexanoic acid

Details on test material:

Purity: 100%
Lot No. OP 2/94
brown, viscous liquid
Mp -20 to -30 deg C
Bp 185 deg C
Water solubility: ambigous results
Methanol solubility: >100 g/L

Radiolabelling:

no

Sampling and analysis

Details on sampling:

Sampling twice a week, in total 16 times for determination of substance concentration in water. 4 Samplings for the analysis of the test itemin fish (after 2, 4, 6 and 8 weeks after intial exposure (0 d). Sampling in control fish at begin and end of the test

Test solutions

Vehicle:

yes

Details on preparation of test solutions, spiked fish food or sediment:

Dsipersant HCO-40, test item and 20 times its volume of HCO-40 were kneaded together preparing a stock solution by dilution with ion exchange water of 2000 mg/L

Test organisms

Test organisms (species):

Cyprinus carpio

Details on test organisms:

Supply: Sugishima Fish Hatchery
Rearing for two days to remove fish with abnormalities, Acclimatization at 25 +/- 2 deg C applying a drug to remove parasites, afterwards rearing for 57d in flowing water
Body weight: average 25.2 g
Body length: 9.9 cm
Lipid content: average 3.5%
Feed: pellet shaped compound feed for carp
2 concentration groups with 11 fish at start of exposure
Control group: 5 fish

Study design

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

57 d

Test conditions

Hardness:

not given

Test temperature:

25 +/- 2 deg C

pH:

not given

Dissolved oxygen:

7.0 to 7.6 mg/L

TOC:

not given

Details on test conditions:

Quantity of test water: 579 L/d was supplied at 2 ml / min, 100l glas water tanks

Nominal and measured concentrations:

1st concentration group: 0.6 mg/L
2nd concentration group: 0.06 mg/L

Details on estimation of bioconcentration:

Measurement of the test item in the test water (sampling see above) and measurement in fish. Whole fish was cut in pieces and extracted with acetonitrile / phosphoric acid after homogenization. After the workup procedure, the test item was determinded with HPLC (see above)

Results and discussion

Bioaccumulation factor

Bioaccumulation factor 1

Type:

BCF

Value:

8.6 L/kg

Basis:

whole body w.w.

Time of plateau:

14 d

Calculation basis:

steady state

Remarks on result:

other: test item conc. in fish LODm (LOQm = 3*LODm)

Remarks:

Conc.in environment / dose:0.6 mg/L (Group 1)

Bioaccumulation factor 2

Type:

BCF

Value:

< 15 L/kg

Basis:

whole body w.w.

Time of plateau:

14 d

Calculation basis:

steady state

Remarks on result:

other: Min. BCF (Group 1) based on the LOQm test item in fish and measured test item conc. in water is BCF=15

Remarks:

Conc.in environment / dose:0.6 mg/L (Group 1)

Bioaccumulation factor 3

Type:

BCF

Value:

25 L/kg

Basis:

whole body w.w.

Time of plateau:

14 d

Calculation basis:

steady state

Remarks on result:

other: test item conc. in fish is < LODm

Remarks:

Conc.in environment / dose:0.06 mg/L (Group 2)

Bioaccumulation factor 4

Type:

BCF

Value:

< 158 L/kg

Basis:

whole body w.w.

Time of plateau:

14 d

Calculation basis:

steady state

Remarks on result:

other: Min. BCF (Group 2) based on the LOQm test item in fish and measured test item conc. in water is BCF=158

Remarks:

Conc.in environment / dose:0.06 mg/L (Group 2)

Details on kinetic parameters:

none available

Metabolites:

not monitored

Results with reference substance (positive control):

no reference substance used

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Tetra-PSCA has shown a low bioaccumulation potential in an 8 week Fish BCF test. Most concentrations in the fish were below the Limit of Quantification of the Analytical method (LOQ m) or even below the LOD m (Limit of Detection). Two concentrations of the test item in water were tested (0.6 and 0.06 mg/L) and of each group one BCF could be calculated: BCF=8.6 L/kg wwt for the concentration group 1 (water conc. 0.6 mg/L) and BCF=25 L/kg wwt for concentration group 2 (water conc. 0.06 mg/L). These both BCF are below the minimum BCF which can be calculated from the LOQm for test item concentration in fish which is 8.9 µg/g and the test water concentration of the two groups. The minimum BCF which can be reliably calculated are 15 L/kg wwt for Group 1 (water conc. 0.6 mg/L) respectively 158 L/kg wwt for Group 2 (water conc. 0.06 mg/L). This means that the BCF (Group 1) is < 15 L/kg wwt and <158 L/kg wwt (Group 2) as no fish concentrations were measured in this test which would result in a higher BCF as these minimum BCF.