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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1980
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Results issued from reviewed document but only a brief summary was available.

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1980

Materials and methods

Principles of method if other than guideline:
The method was not described.
GLP compliance:
not specified
Test type:
standard acute method

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Name of the test substance (as cited in the test report) : 1,1,2-trichloroethane.

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male
Details on test animals and environmental conditions:
O.F.A. Iffa-Crédo (130-200g body weight)

Administration / exposure

Route of administration:
inhalation
Type of inhalation exposure:
whole body
Vehicle:
other: no data
Details on inhalation exposure:
Each independent exposure chamber received a conditioned laminar air flow (24± 1 °C, 50± 2 % relative humidity). The air flow to each exposure chamber was approximately 12 m3/h. The exposure chambers were in a constant under-pressure of about 3mm water column. Micropumps with a variable volume conveyed the 1,1,2-trichloroethane to the injector located near the entrance of the exposure chamber. The injector vaporized the 1,1,2-trichloroehtane by heating controlled by a thermostat. Rats were only introduced into the exposure chamber (using an air-lock) when the targeted concentration was reached.

Analytical verification of test atmosphere concentrations:
yes
Remarks:
Samples of the test atmosphere were collected regularly (every 3 min.) from the exposure chamber and analyzed using gas chromatography.
Duration of exposure:
6 h
Concentrations:
Geometric series (factor 1.05-1.1). Concentration range was set based on a range-finding experiment using 3 rats/dose and a geometric concentration series (factor 2)
No. of animals per sex per dose:
12
Control animals:
not specified
Details on study design:
A range-finding study (3 rats/dose) preceded the main study. In the rang-finding study the concentrations were set according to a geometric series (factor 2). In the main experiment (12 rats/dose) the concentrations were set according to a geometric series (factor 1.05-1.1). Rats were exposed during 6h and the follow-up period was 14 days. There rats were frequently observed during exposure. Rats were weighed before exposure, and on day 7 and 14 post-exposure. A macroscopic evaluation of the lung, liver and kidneys was performed on the rats surviving the 14 day exposure period. In case of abnormal findings tissues were collected for microscopic evaluation.
Statistics:
The LD50 values and the 95% confidence interval were calculated according to the method of Bliss (Quart. U. Pharm. Pharmacol, 1938,2, 192-216). Weighted Linear regression with log-transformed concentrations on X-axis, and probit-transformed lethality on Y-axis.

Results and discussion

Effect levels
Sex:
male
Dose descriptor:
LC50
Effect level:
9 000 mg/m³ air
Exp. duration:
6 h
Mortality:
Mortality was primarily observed during the 6h exposure period or during the first 24h following exposure. Some mortality was still observed between the 2nd till the 8th day post-exposure.
Clinical signs:
exitation, somnolent
Body weight:
A reduction of the body weigt gain was observed.
Gross pathology:
none of the rats surviving the 14 day follow-up period showed abnormal macroscopical findings.
Other findings:
-

Any other information on results incl. tables

The LC50 was reported to be 1654 ppm, the 95% confidence interval being 1586 -1725 (equivalent to 9 g/m3, 95% confidence interval: 8.6 -9.4 ).

Applicant's summary and conclusion

Interpretation of results:
harmful
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
The LC50 with rats exposed for 6 hours was reported to be 9 g/m3.
Executive summary:

The LC50 with Sprague Dawley male rats exposed for 6 hours was reported to be 9 g/m3.