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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
1988
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: Study performed according to standardised protocol, but test conditions and results were not sufficiently detailed.

Data source

Reference
Reference Type:
publication
Title:
Rat liver foci and in vitro assays to detect initiating and promoting effects of chlorinated ethanes and ethylenes
Author:
Milman, H.A., Story, D.L., Riccio, E.S., Sivak, A., Tu, A.S. Williams, G.M., Tong, C., Tyson, C.A.
Year:
1988
Bibliographic source:
Ann. N.Y. Acad. Sci., 534: 521-530.

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
Deviations:
yes
Remarks:
Test results and methods not sufficiently detailed
Principles of method if other than guideline:
Ames test: direct plate-test
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 1,1,2-trichloroethane
- 97-99% purity
- Origin: Aldrich Chemical Co.

Method

Target gene:
histidine reverse
Species / strain
Species / strain:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Details on mammalian cell lines (if applicable):
not applicable
Additional strain characteristics:
not specified
Metabolic activation:
with and without
Metabolic activation system:
S-9 fraction from Aroclor 1254-induced livers derived from Osborne Mendel rats or B6C3F1 mice of both sexes.
Test concentrations with justification for top dose:
no data
Controls
Negative controls:
not specified
Solvent controls:
not specified
True negative controls:
not specified
Positive controls:
not specified
Positive control substance:
not specified

Results and discussion

Test results
Species / strain:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity:
not specified
Vehicle controls valid:
not specified
Negative controls valid:
not specified
Positive controls valid:
not specified
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

In the same publication, positive results on strains TA1535 ad TA100 were reported for other tested compounds, i.e. Etylene chloride, 1,1-dichloroethane, 1,2-dichloroethane and trichloroethylene.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative

The test substance was found to be negative in this Ames test.
Executive summary:

The test substance was found to be negative in this Ames test. Testing was performed on S. typhimurium TA 1535, TA 1537, TA 98 and TA 100 with and without S9.