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EC number: 201-166-9 | CAS number: 79-00-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Basic toxicokinetics
Administrative data
- Endpoint:
- basic toxicokinetics
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1971
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study well documented, meets generally accepted scientific principles, performed in a way similar to OCDE 417 Guidelines, with acceptable restrictions.
Data source
Reference
- Reference Type:
- publication
- Title:
- Metabolism of 1,1,2-trichloroethane 1,2-14C in the Mouse
- Author:
- Yllner S
- Year:
- 1 971
- Bibliographic source:
- Acta Pharmacol. et Toxicol. 1971, 30, 248-256
Materials and methods
- Objective of study:
- metabolism
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 417 (Toxicokinetics)
- Deviations:
- yes
- Remarks:
- Only single administration was tested; justification of dose spacing is not reported
- Principles of method if other than guideline:
- The labelled trichloroethane was administered by intraperitoneal injection as a 10% solution in olive oil. The animals were placed in chamber with flow of dry air free of carbon dioxide. The elimination of radioactivity was followed for 3 days (urine, faeces and volatile metabolite). Isotope dilution analysis was performed for identification and determination of metabolites.
- GLP compliance:
- no
Test material
- Reference substance name:
- 1,1,2-trichloroethane
- EC Number:
- 201-166-9
- EC Name:
- 1,1,2-trichloroethane
- Cas Number:
- 79-00-5
- Molecular formula:
- C2H3Cl3
- IUPAC Name:
- 1,1,2-trichloroethane
- Details on test material:
- - Name of test material: 1,1,2-trichloroethane
- Analytical purity: approximately 97%
- Impurities: two peaks of impurity, one of which was possibly tetrachloroethylene, the other was not identified.
Radiolabelled compound:
- Radiochemical purity (if radiolabelling): 96.3%
- Impurities (identity and concentrations): inactive 1,1,2 trichloroethane and 1,1,2,2 tetrachloroethane
- Specific activity (if radiolabelling): 0.38 µCi/mg
- Locations of the label (if radiolabelling): 1,1,2-trichloroethane-1,2-14C. Labelled 1,1,2-trichloroethane prepared by chlorination of ethylene 14C.
- Storage condition of test material: condensed in U-tube and cooled at -80°C.
- Source: The radiochemical centre; Amersham
Constituent 1
- Radiolabelling:
- yes
Test animals
- Species:
- mouse
- Strain:
- other: Albino
- Sex:
- female
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- olive oil
- Duration and frequency of treatment / exposure:
- single i.p. injection
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0.10, 0.13, 0.14 and 0.19 g/kg (10% in olive oil)
- No. of animals per sex per dose / concentration:
- 1 female at 0.10, 0.13 and 0.19 g/kg
2 females at 0.14 g/kg - Control animals:
- no
Results and discussion
- Preliminary studies:
- 1,1,2-trichloroethane was dissolved by addition of alcohol in phosphate buffer (pH 7.2) also containing cysteine. Under these conditions, 1,1,2-trichloroetheane was found to be stable. Determinations of trichloroethane and cysteine in samples of the solutions disclosed no consumption of these compounds during 24 hours
Toxicokinetic / pharmacokinetic studies
- Details on absorption:
- no data
- Details on distribution in tissues:
- no data
- Details on excretion:
- - During the first 24 hours, more than 90% of the activity (82-98 %) was excreted, mainly in the urine (about 76.4% of the administered radioactivity was recovered in urines at 24 hours following administration).
- At 72 hours, about 7 per cent of the activity ( 2-10 %) was recovered in traps 1 and 2 and the oxidation to respiratory carbon dioxide was equivalent to 12 % (8-15 %) of the dose. Less than 1% was found in the faeces, and this activity was probably due to contamination with the urine. Only 2% still remained in the mice after 3 days.
Metabolite characterisation studies
- Metabolites identified:
- yes
- Details on metabolites:
- Examination of the urine by paper chromatograpy showed 3 major metabolites and their urinary activity were estimated by isotope dilution analysis:
1- chloracetic acid (6-31%)
2- S-carboxymethylcysteine (free: 29-46%) and (conjugated: 3-10%)
3- thio-diacetic acid (38-42%)
and small amounts 2,2-dichloroethanol, 2,2,2-trichloroethanol, oxalic acid and trichloroacetic acid
The percentage distribution of urinary metabolites of mice receiving chloroacetic 1-14 C acid is shown for comparison (see Table1)
Any other information on results incl. tables
Table 1: Isotope dilution analysis of urinary metabolites from mice receiving 1,1,2 trichloroethane (1,2-14 C). Percentage of urinary activity excreted in 24 hours.
The percentage distribution of urinary metabolites of mice receiving chloroacetic1 -14C acid is shown for comparison.
Mean |
Range |
Metabolites of chloroacetate |
|
Chloroacetic acid |
16(3) |
6-31 |
13 |
S-carboxymetlycysteine |
38 (3) |
29-46 |
39 |
Conjugated s-carboxymetlycysteine |
5 (3) |
3-10 |
3 |
Thiodiacetic acid |
40 (2) |
38-42 |
37 |
2,2 dichloroethanol |
1.4 (3) |
0.9-2.1 |
- |
Oxalic acid |
0.4 (2) |
0.3-0.5 |
0.2 |
Trichloroacetic acid |
1.9 (3) |
1.4-2.3 |
- |
2,2,2 trichloroethanol |
0.2 (2) |
- |
- The figures in brackets are the number of animals
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): no bioaccumulation potential based on study results
Under these test conditions, test substance was almost completely excreted 3 day after the i.p. injection. In comparison with the results obtained by the administration of chloroacetic 1-14C-acid in mouse (Yllner, 1970), it is suggested that the metabolism of 1,1,2-trichloroethane proceeds via chloroacetic acid. - Executive summary:
Labelled 1,1,2-trichloroethane (0.38 µci/mg), prepared by chlorination of ethylene -14C, was injected intraperitoneally (0.1 - 0.2 g/kg). The elimination of radioactivity was followed for 3 days. 73 - 87% of the dose was found in the urine, 0.1 - 2% in the faeces (contaminated by urine) and 16 -22% in the expired air. About 3/5 of the expired activity was due to carbon dioxide and the remainder to unchanged trichloroethane. 1 -3% of the dose remained in the animal. Examination of the urine by paper chromatography showed 3 major metabolites and there were estimated by isotope dilution analysis: chloroacetic acid (6 -31%), s-carboxymethylcysteine (29 -46% (free) and 3 -10 (conjugated)) and thio-diacetic acid (38 -42). These results are in good agreement with those obtained by the administration of chloroacetic1-14C-acid. It may therefore be suggested that the metabolism of 1,1,2-trichloroethane proceeds mainly via chloroacetic acid.
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