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Environmental fate & pathways

Biodegradation in water and sediment: simulation tests

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Endpoint:
biodegradation in water: sewage treatment simulation testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2013-2014
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Well conducted GLP study
Qualifier:
according to
Guideline:
OECD Guideline 303 A (Simulation Test - Aerobic Sewage Treatment. A: Activated Sludge Units)
Deviations:
yes
Remarks:
As the test item was shown to be extremely volatile under aeration in the preliminary investigational work, no further work will be conducted for this study as aeration is needed for this study type.
Principles of method if other than guideline:
This report contains a description of the methods used and results obtained during preliminary investigational work to investigate the volatility of the test item when exposed to aerobic conditions. The results of the preliminary investigational work are of value in the assessment of the volatility
of the test item in the aquatic environment, and the suitability of the proposed method of
accessing the biodegradation of the test item.
GLP compliance:
yes (incl. certificate)
Oxygen conditions:
aerobic
Inoculum or test system:
other: reverse osmosis water
Remarks:
preliminary scoping of technical feasibility due to substance volatility
Details on source and properties of surface water:
Not applicable
Details on source and properties of sediment:
Not applicable
Details on inoculum:
Not applicale
Details on study design:
Preliminary Investigational Work:
Information provided by the Sponsor indicated that the test item was extremely volatile.
Therefore preliminary investigational work was performed in order to investigate this.
An amount oftest item (100 mg) was dissolved in reverse osmosis water (1 liter) with the aid of
shaking by hand for approximately 1 minute and sonication for 6 minutes to give a 100 mg/L
stock solution. One untreated sample was taken for DOC analysis. This stock solution was then
subjected to aeration (1.0 Llmin) for 24 hours in darkness and a further untreated sample taken
for DOC analysis. A control sample containing reverse osmosis water was prepared to correct
the DOC values.
Due to the light sensitive nature of the test item once formulated the volumetric flask containing
the stock solution was shielded from light with aluminum foil.
The samples were analyzed for DOC using a Shimadzu TOC-VcsH TOC analyzer
Test performance:
The results obtained from the samples taken for DOC analysis from the preliminary investigational work indicated that the test item was extremely volatile in solution when being subjected to aeration. The failure to achieve a near nominal carbon content prior to aeration indicated significant losses
of test item through volatilization occurred during the initial preparation of the stock solution,
further confirming the unsuitability of the test item for this particular study type.
% Degr.:
0
Parameter:
not specified
Sampling time:
0 min
Remarks on result:
other: substance too volatile for reliable test to be conducted
Compartment:
other:
DT50:
ca. 0 min
Type:
not specified
Remarks on result:
other: substance too volatile for reliable test to be conducted
Transformation products:
not specified
Details on results:
The results obtained from the samples taken for DOC analysis from the preliminary investigational work indicated that the test item was extremely volatile in solution when being subjected to aeration. The failure to achieve a near nominal carbon content prior to aeration indicated significant losses
of test item through volatilization occurred during the initial preparation of the stock solution,
further confirming the unsuitability of the test item for this particular study type.
Results with reference substance:
Not applicable.

The results obtained from the samples taken for DOC analysis from the preliminary investigational work indicated that the test item was extremely volatile in solution when being subjected to aeration. The failure to achieve a near nominal carbon content prior to aeration indicated significant losses of test item through volatilization occurred during the initial preparation of the stock solution, further confirming the unsuitability of the test item for this particular study type.

Validity criteria fulfilled:
not applicable
Conclusions:
The results obtained from the samples taken for DOC analysis from the preliminary investigational work indicated that the test item was extremely volatile in solution when being subjected to aeration (see Table 1).
The failure to achieve a near nominal carbon content prior to aeration indicated significant losses
of test item through volatilization occurred during the initial preparation of the stock solution,
further confirming the unsuitability of the test item for this particular study type.
Executive summary:

This report contains a description of the methods used and results obtained during preliminary investigational work to investigate the volatility of the test item when exposed to aerobic conditions. The results of the preliminary investigational work are of value in the assessment of the volatility of the test item in the aquatic environment, and the suitability of the proposed method of accessing the biodegradation of the test item.

The results obtained from the samples taken for DOC analysis from the preliminary investigational work indicated that the test item was extremely volatile in solution when being subjected to aeration (see Table 1). The failure to achieve a near nominal carbon content prior to aeration indicated significant losses of test item through volatilization occurred during the initial preparation of the stock solution, further confirming the unsuitability of the test item for this particular study type.

The preliminary investigational work conducted confirmed that the test item was extremely volatile when subject to aeration and under the test conditions that are employed for this study type this study would not be suitable for this test item.

Endpoint:
biodegradation in water: simulation testing on ultimate degradation in surface water
Data waiving:
study technically not feasible
Justification for data waiving:
other:
Endpoint:
biodegradation in water: sediment simulation testing
Data waiving:
study technically not feasible
Justification for data waiving:
other:

Description of key information

Preliminary investigational work for an OECD 303A test is available, showing that the test substance is too volatile to be analysed in this test.

Key value for chemical safety assessment

Additional information

The results obtained from the samples taken for DOC analysis from the preliminary investigational work indicated that the test item was extremely volatile in solution when being subjected to aeration. The failure to achieve a near nominal carbon content prior to aeration indicated significant losses of test item through volatilization occurred during the initial preparation of the stock solution, further confirming the unsuitability of the test item for this particular study type.