Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 203-733-6
CAS number: 110-05-4
Guideline suitably followed and relevant quality criteria reported
except temperature. Study was conducted under GLP. Although no
certificate of analysis was included purity and batch number was
reported. Information over temperature and calculation of theoretical
oxygen demand is lacking. Test was conducted only in duplicate and a
higher degradability might be expected with more replicate.
In order to assess the biotic degradation, a ready biodegradability test
was performed which allows the biodegradability to be measured in an
aerobic aqueous medium. The ready biodegradability was determined in the
closed bottle test performed according to slightly modified OECD 301D,
EEC and ISO Test Guidelines. Microorganisms (Secondary activated sludge
at concentration of 2 mg DW/L, obtained from the RZWI Nieuwgraaf in
Duiven (1989.09.01): plant treating predominantly domestic wastewater)
are inoculated into a chemically defined liquid medium containing the
test substance (Di-tert-butyl at 2 mg/L) or the reference substance
(Acetic acid, sodium salt or Sodium acetate at 6.7 mg/L) under aerobic
conditions for a period of 98 days; the test was prolonged because the
pass level was not reached at day 28. Use was made of 2 replicates for
blank control (without test material and inoculum), for blank inoculum
(without test material but with inoculum), for test material with
inoculum, and for reference substance with inoculum. The pH was 6.7 at
the end of the test period. Parameter followed for degradation
estimation was O2 consumption. The dissolved oxygen concentrations were
determined electrochemically using a oxygen electrode and meter at days
0, 5, 15, 28, 42, 56, 71 and 98.
The test substance caused no reduction in the endogenous respiration.
Therefore, Di-tert-butyl is considered to be non-inhibitory to the
inoculum. Di-tert-butyl was biodegraded 6% at day 28 under the stringent
conditions in the close bottle test and, therefore should not be
classified as ready biodegradable. This study was carried out under GLP
and fulfilled validity criteria. Some minor deviations to the guidelines
were justified but the temperature was not reported and some
informations are scarce.
The substance is not readily biodegradable in a ready biodegradation study.
biotic degradability of the substance was assessed in a GLP study
according to OECD 301D, close bottle test (Ginkel, 1990). The study is
reliable and found no significant biodegradation (6%) after 28 days
while no inhibition was observed and the reference substance was
successfully degraded. The study was then prolonged to 98 days without
further biodegradation. It is finally concluded the substance is not
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again