Registration Dossier
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EC number: 202-764-2 | CAS number: 99-54-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
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- Vapour pressure
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- Endpoint summary
- Stability
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- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
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- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Endpoint summary
Administrative data
Key value for chemical safety assessment
Effects on fertility
Description of key information
During 28-day-treatment of male and female Wistar rats with 0, 4, 20 or 100 mg/kg bw/d 1,2 -dichloro-4 -nitrobenzene dissolved in sesame oil according to OECD TG 407 reproductive organs were not affected (Hoechst AG 1993).
Effect on fertility: via oral route
- Dose descriptor:
- NOAEL
- 100 mg/kg bw/day
Additional information
As confirmed by literature (e.g. Mangelsdorf I. et al (2003) Regul. Toxicol. Pharmacol. 37, 356 -369) histopathologogical examinations in repeated dose toxicity studies are of high value and of high sensitivity for the evaluation of reproductive toxicity. Also, it is agreed that histopathological changes on the reproductive organs in repeated dose toxicity studies are indicative of effects on fertility. In the above cited study no effects were observed in the reproductive organs up to the highest used dose of 100 mg/kg bw/day although clear systemic toxicity is given.Therefore no further testing should be required.
Short description of key information:
Studies dealing specifically with toxicity to reproduction were not identified.
During 28-day-treatment of male and female Wistar rats with 0, 4, 20 or 100 mg/kg bw/d 1,2 -dichloro-4 -nitrobenzene dissolved in sesame oil according to OECD TG 407 reproductive organs were not affected despite clear systemic toxicity up to the maximum tolerated dose of 100 mg/kg bw/day (Hoechst AG 1993 cited in UNEP 2005, see also toxicity to reproduction: other studies). Therefore there is no evidence of toxicity to the reproductive organs.
Effects on developmental toxicity
Description of key information
Pregnant female Sprague-Dawley rats were orally applied with 0, 10, 30, or 100 mg/kg bw/day 1,2 -dichloro-4-nitrobenzene dissolved in corn oil by gavage resulting in developmental effects (significant iincrease in dilated ureters) in the presence of maternal toxicity (significantly reduced body weight gain on gd 6 -10). The NOAEL for maternal toxicity and developmental toxicity is 10 mg/kg bw/d (Monsanto 1987)
Effect on developmental toxicity: via oral route
- Dose descriptor:
- NOAEL
- 10 mg/kg bw/day
Additional information
Pregnant female Sprague-Dawley rats were orally applied with 0, 10, 30, or 100 mg/kg bw/day 1,2 -dichloro-4-nitrobenzene dissolved in corn oil by gavage resulting in developmental effects (significant iincrease in dilated ureters) in the presence of maternal toxicity (significantly reduced body weight gain on gd 6 -10). The NOAEL for maternal toxicity and developmental toxicity is 10 mg/kg bw/d (Monsanto 1987)
Toxicity to reproduction: other studies
Additional information
During 28-day-treatment of male and female Wistar rats with 0, 4, 20 or 100 mg/kg bw/d 1,2 -dichloro-4 -nitrobenzene dissolved in sesame oil according to OECD TG 407 reproductive organs were not affected (Hoechst AG 1993).
Justification for classification or non-classification
Fertility
Due to the above discussion no classification with respect to fertility assessment has to be included.
Developmental toxicity
The available developmental toxicity study shows that developmental toxic effects occur only in the presence of maternal toxicity. Thus, there is no need for classification with respect to developmental toxicity.
Additional information
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