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EC number: 203-825-6
CAS number: 111-01-3
The ready biodegradation of the squalane was determined by the carbon
dioxide evolution test method (OCDE guideline 301B). Tests of ready
biodegradability are stringent tests that provide limited oppotunity for
acclimation and biodegradation to occur. In the CO2 test, inoculated
mineral medium was dosed with a known amount of test substance as the
nominal sole source of organic carbon and aerated with CO2 -free air.
The CO2 produced from the mineralization of organic carbon within the
test chambers was displaced by the flow of CO2 -free air and trapped as
K2CO3 in KOH trapping solution. The amount of CO2 produced by the test
substance is expressed as a percentage of the theoretical amount of CO2
that could have been produced if complete biodegradation of the
substance occured. The test contained a blank control, three reference
groups and one treatment group. Each group contained two replicate test
chambers. The blank control was used to meseare the background CO2
production of the inoculum and was dosed with a carbon source. The
reference chambers were dosed with either canola oil, Synfluid or Ultra
Low Sulfur Diesel at a concentration of 10 mg C/L. The treatment group
test chambers were used to evaluate the test substance at concentration
of approximately 10 mg C/L. The results indicated that the activated
sludge inoculum was active, degrading the canola oil reference 99.9%.
The average cumulative percent biodegradation for squalane was 64.7%.
However, squalane may be considered inherently biodegradable because it
reached 60% of TCO2, though not within a 10 -days window of reaching 10%
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