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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2013

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: liquid

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
LC0
Effect conc.:
> 100 mg/L
Conc. based on:
other: loading rate

Any other information on results incl. tables

In the analyzed test medium samples from the start of the test medium renewal periods the measured test item concentration ranged between 2.1 and 12 μg/L. During the test medium renewal period of 24 hours the test item concentration in the test medium decreased. At the end of the test medium renewal periods, the test item concentration ranged between 1.4 and 2.7 μg/L. The mean measured concentration during the test period was calculated to be 3.8 μg/L (calculated as the arithmetic mean of the two geometric means determined for two test medium renewal periods).

The biological results were related to the loading rate of 100 mg/L and to the mean measured test concentration of 3.8 μg/L. In the control and at the loading rate of 100 mg/L (mean measured 3.8 μg/L), no immobilized test organisms were observed during the test period of 48 hours. Therefore, the 48-hour NOEC and EC0 of Squalane (CAS 111-01-3) to Daphnia magna were determined to be at least at the loading rate of 100 mg/L (mean measured 3.8 μg/L). The 48-hour NOEC and EC0 might even be higher, but loading rates above 100 mg/L were not tested, in accordance with the test guidelines. The 48-hour EC50 and EC100 were clearly higher than the loading rate 100 mg/L (mean measured 3.8 μg/L).

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
In conclusion, the test item Squalane (CAS 111-01-3) had no toxic effects on Daphnia magna during the 48-hour test period up to a loading rate of 100 mg/L (mean measured concentration of 3.8 μg/L) respectively up to its solubility limit in the test water under the present test conditions.
Executive summary:

The acute toxicity of the test item Squalane (CAS 111-01-3) to Daphnia magna was determined in a 48-hour semi-static test according to the EU Commission Directive 92/69/EEC, Part C.2, the Commission Regulation (EC) No. 440/2008, Part C.2 and the OECD Guideline for Testing of Chemicals, No. 202 (2004). A limit test was performed in accordance with the test guidelines to demonstrate that the test item has no toxic effect on the test organisms up to the loading rate of 100 mg/L. Due to the low water solubility of the test item, a dispersion of the test item with the loading rate of 100 mg/L was continuously stirred at room temperature in the dark over 96 hours. Then, the dispersion was filtered through a membrane filter (pore size 0.45 μm). The undiluted filtrate of the dispersion was used as the test medium. Additionally, a control was tested in parallel. A semi-static test with test medium renewal after 24 hours was performed to keep the concentration of the test item in the test medium as constant as possible during the test period of 48 hours. Since the test item was supposed to be volatile, the test was performed in a closed system as a pre-caution. The preparation of the test medium was based on the OECD Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures, 2000. In the analyzed test medium samples from the start of the test medium renewal periods the measured test item concentration ranged between 2.1 and 12 μg/L. At the end of the test medium renewal periods, the test item concentration ranged between 1.4 and 2.7 μg/L. The mean measured concentration during the test period was calculated to be 3.8 μg/L. The biological results were related to the loading rate of 100 mg/L and to the mean measured test item concentration of 3.8 μg/L. In the control and at the loading rate of 100 mg/L (mean measured concentration of 3.8 μg/L) no immobilized daphnids were determined during the test period of 48 hours. In conclusion, the test item Squalane (CAS 111-01-3) had no toxic effects on Daphnia magna during the 48-hour test period up to a loading rate of 100 mg/L (mean measured concentration of 3.8°μg/L) and therefore up to its solubility limit in the test water under the present test conditions.