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EC number: 946-188-6
CAS number: -
Algal cells aggregated at concentrations equal or greater than 0.10
mg/L. Consequently, algal cell density measurements based on cell counts
might have been unreliable.
The effect of the test item on algae was assessed in a GLP and guideline
study (OECD testing guideline 201) using the unicellular green alga
Pseudokirchneriella subcapitata (formerly known as Selenastrum
capricornutum), over an exposure period of 72 hours. A test item
specific analytical method could not be develped for the relevant
concentration range of this study. Therefore it was considered
appropriate to calculate the results based on nominal test
concentrations. All test and control cultures were inspected
microscopically at 72 hours. After 72 hours there were no abnormalities
detected in the control or test cultures at 0.032 mg/L. However,
aggregated cells were observed to be present in the test cultures at
0.10, 0.32, 1.0 and 3.2 mg/ L. The EC50(growth rate) was determined as
1.8 mg/L (95% confidence limits could not be determined because the
values did not fit the models available for calculation), the EC50
(yield) was 0.61 mg/L (95% CI 0.50 - 0.75 mg/L). The NOEC (growth rate)
was 0.32 mg/L, with the LOEC at 1.0 mg/L; the NOEC (yield) was 0.10 mg/L
with the LOEC at 0.32 mg/L.
The growth rate of P. tricornutum in CB added f/2 medium did not change when the concentration of CB varied from 100 µM to 300 µM.
CB reduced the growth rate of P. tricornutum in f/2 medium at concentrations of 200 and 400 µM. 100 µM were without effect.
Key values are taken from a GLP study performed in accordance with OECD
test guideline 201, EU method C3 and US EPA procedure OCSPP 850.5400
A study was performed to assess the effect of the test item on the growth of the green alga Pseudokirchneriella subcapitata over an exposure period of 72 hours.
With respect to the inhibitory effect of the test item, the 0-72 h average specific growth rates were significantly different from that of the control group in the examined concentration range of 1.0 to 3.2 mg/L (nominal). The NOEC was determined as 0.32 mg/L (nominal) and the LOEC was determined as 1.0 mg/L (nominal). The 0-72 h areas and yield were significantly different from that of the control group in the examined nominal concentration range of 0.32 to 3.2 mg/L (nominal). The NOEC was determined as 0.10 mg/L (nominal) and the LOEC was determined as 0.32 mg/L (nominal).
The EC50 for growth rate was 1.8 mg/L, and for yield 0.61 mg/L. At any concentrations equal or greater 0.10 mg/L chuffed and aggregated cells were observed.
Exposure over 11 days to nominal concentrations of 100 mg/L or higher of CB, CB or CB had however no effects on the growth rate of Phaeodactylus tricornutum in an earlier, non-GLP study suggesting that nutrient depletion may have caused the effects in the GLP study.
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