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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1998
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
1984
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
The concentration of the test substance in the test solution was measured at the beginning and the end of the exposure. No further details.
Vehicle:
not specified
Test organisms (species):
Daphnia magna
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
20 ± 1 ºC
Nominal and measured concentrations:
0 (control), 32, 56, 100, 180, 320 mg/L (nominal concentrations)
Details on test conditions:
TEST SYSTEM
- Material, size, headspace, fill volume: 100 mL
- Renewal rate of test solution (frequency/flow rate): no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

OTHER TEST CONDITIONS
- Adjustment of pH: not specified
- Photoperiod: 16 hours light - 8 hours dark (room light)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility
Reference substance (positive control):
not specified
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
210 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
230 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
95 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Validity criteria fulfilled:
not specified
Conclusions:
The 48h-EC50 of the test item to daphnia is 210 mg/L.
Executive summary:

An acute toxicity test was performed on Daphnia magna according to OECD 202, under GLP conditions. 20 daphnids per concentration (4 vessels with 5 animals each) were exposed to 0 (control), 32, 56, 100, 180, 320 mg/L (nominal concentrations) of test item for 48h at 20 ± 1 ºC, under static conditions. The test item concentration was measured at the beginning and at the end of the exposure time. Based on the test results, the 48 h-EC50 of the test item in daphnia was determined to be 210 mg/L, and the NOEC was 95 mg/L, both based on mobility (measured concentrations).

Description of key information

Key study. Test according to OECD 202, GLP study. The 48 h-EC50 of the test item in daphnia was determined to be 210 mg/L, and the NOEC was 95 mg/L, both based on mobility (measured concentrations).

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
210 mg/L

Additional information