Toluene-2-sulphonamide

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1998

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

1984

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

The concentration of the test substance in the test solution was measured at the beginning and the end of the exposure. No further details.

Vehicle:

not specified

Test organisms (species):

Daphnia magna

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

20 ± 1 ºC

Nominal and measured concentrations:

0 (control), 32, 56, 100, 180, 320 mg/L (nominal concentrations)

Details on test conditions:

TEST SYSTEM
- Material, size, headspace, fill volume: 100 mL
- Renewal rate of test solution (frequency/flow rate): no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

OTHER TEST CONDITIONS
- Adjustment of pH: not specified
- Photoperiod: 16 hours light - 8 hours dark (room light)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility

Reference substance (positive control):

not specified

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

210 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

230 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

95 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

not specified

Conclusions:

The 48h-EC50 of the test item to daphnia is 210 mg/L.

Executive summary:

An acute toxicity test was performed on Daphnia magna according to OECD 202, under GLP conditions. 20 daphnids per concentration (4 vessels with 5 animals each) were exposed to 0 (control), 32, 56, 100, 180, 320 mg/L (nominal concentrations) of test item for 48h at 20 ± 1 ºC, under static conditions. The test item concentration was measured at the beginning and at the end of the exposure time. Based on the test results, the 48 h-EC50 of the test item in daphnia was determined to be 210 mg/L, and the NOEC was 95 mg/L, both based on mobility (measured concentrations).

Description of key information

Key study. Test according to OECD 202, GLP study. The 48 h-EC50 of the test item in daphnia was determined to be 210 mg/L, and the NOEC was 95 mg/L, both based on mobility (measured concentrations).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

210 mg/L