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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1998
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
The concentration of the test substance in the test solution was measured at the beginning and the end of exposure. No further details.
Vehicle:
not specified
Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Common name: algae
- Strain: ATCC 22662
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Test temperature:
23 ± 2ºC
Nominal and measured concentrations:
- Nominal: 0 (control); 3.2, 10, 32, 100 and 320 mg/L
- Measured (0 h): 0, 2.4, 7.4, 27, 78 and 260 mg 1 L
Details on test conditions:
TEST SYSTEM
- Material, size, headspace, fill volume: Shake culture (100 rpm), 100 mL (OECD medium)
- Initial cells density: 1×10^4cells/mL
- No. of vessels per concentration (replicates): 3

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: continuous lighting
- Light intensity and quality: 4000 to 5000 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : growth inhibition, area under the curve
Reference substance (positive control):
not specified
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
170 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
7.4 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
57 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
other: AUC
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
7.4 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
other: AUC
Details on results:
- Growth rate values: calculated from original data because 0-72 hr toxicity value of Alga growth rate was not reported on the report before 2003.
Validity criteria fulfilled:
not specified
Conclusions:
Based on the growth rate, the 72h-EC50 of the test item in algae is 170 mg/L and the 72h-NOEC is 7.4 mg/L.
Executive summary:

An algae growth inhibition test was conducted with the test item in Pseudokirchneriella subcapitata / Selenastratum capricornutum (ATCC 22662), according to the OECD guideline 201, under GLP conditions. Algae were exposed to the test item at concentrations of 0 (control), 3.2, 10, 32, 100 and 320 mg/L for 72 hours at 23 ± 2ºC. The test item concentration was measured by GC-MS at the beginning and at the end of the exposure time and, since the measured concentrations were outside the range ± 20 % of the nominal, all results were given as measured values. Based on the growth rate, the 72h-EC50 of the test item in algae was determined to be 170 mg/L and the 72h-NOEC was 7.4 mg/L (average value of the measured concentration).

Description of key information

Key study. Method according to the OECD guideline 201, GLP study. Based on the growth rate, the 72h-EC50 of the test item in algae was determined to be 170 mg/L and the NOEC was 7.4 mg/L (average value of the measured concentration).

Key value for chemical safety assessment

EC50 for freshwater algae:
170 mg/L
EC10 or NOEC for freshwater algae:
7.4 mg/L

Additional information