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Ecotoxicological information

Toxicity to soil microorganisms

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Description of key information

Pinyl Iso Butyraldehyde was tested for its effect on the activity of soil microflora (nitrogen transformation) according to OECD TG 216 Nitrogen Transformation Test. Nitrogen-transformation in soil enriched with lucerne meal was determined. Test item treated soil was compared with a non-treated soil. NO3 -nitrogen formed in the nitrification process was determined by continuous flow analysis. The following test concentrations were used: 10, 32, 100, 320 and 1000 mg test item/kg soil dw. Sodium chloride was used as reference item. Moisture content was 48% to 49% of WHCmax and temperature was 20°C ± 2°C in the dark.

Results: At day 28 the test item concentration of 10 mg/kg soil dry weight and control was within a range of 25% deviation to control; at 32 mg and 100 mg test item/kg soil dry weight showed no negative effect on the nitrate-N content, but increased nitrate-N contents compared to control, while 320 mg/kg and 1000 mg/kg soil dw had a strong inhibiting effect on the nitrate-N concentrations. Therefore, it can be concluded that the EC50 is between 100 mg and 320 mg test item/kg soil dry weight. The statistical analysis revealed that the NOEC for an inhibition of the nitrogen transformation in soil is 100 mg test item/kg soil dw.

Key value for chemical safety assessment

Long-term EC10 or NOEC for soil microorganisms:
100 mg/kg soil dw

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