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Diss Factsheets

Ecotoxicological information

Toxicity to soil microorganisms

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Administrative data

Link to relevant study record(s)

Description of key information

Pinyl Iso Butyraldehyde was tested for its effect on the activity of soil microflora (nitrogen transformation) according to OECD TG 216 Nitrogen Transformation Test. Nitrogen-transformation in soil enriched with lucerne meal was determined. Test item treated soil was compared with a non-treated soil. NO3 -nitrogen formed in the nitrification process was determined by continuous flow analysis. The following test concentrations were used: 10, 32, 100, 320 and 1000 mg test item/kg soil dw. Sodium chloride was used as reference item. Moisture content was 48% to 49% of WHCmax and temperature was 20°C ± 2°C in the dark.

Results: At day 28 the test item concentration of 10 mg/kg soil dry weight and control was within a range of 25% deviation to control; at 32 mg and 100 mg test item/kg soil dry weight showed no negative effect on the nitrate-N content, but increased nitrate-N contents compared to control, while 320 mg/kg and 1000 mg/kg soil dw had a strong inhibiting effect on the nitrate-N concentrations. Therefore, it can be concluded that the EC50 is between 100 mg and 320 mg test item/kg soil dry weight. The statistical analysis revealed that the NOEC for an inhibition of the nitrogen transformation in soil is 100 mg test item/kg soil dw.

Key value for chemical safety assessment

Long-term EC10 or NOEC for soil microorganisms:
100 mg/kg soil dw

Additional information