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EC number: 251-718-8
CAS number: 33885-52-8
Pinyl Iso Butyraldehyde was tested for its effect on the activity
of soil microflora (nitrogen transformation) according to OECD TG 216
Nitrogen Transformation Test. Nitrogen-transformation in soil enriched
with lucerne meal was determined. Test item treated soil was compared
with a non-treated soil. NO3 -nitrogen formed in the nitrification
process was determined by continuous flow analysis. The following test
concentrations were used: 10, 32, 100, 320 and 1000 mg test item/kg soil
dw. Sodium chloride was used as reference item. Moisture content was 48%
to 49% of WHCmax and temperature was 20°C ± 2°C in the dark.
Results: At day 28 the test item concentration
of 10 mg/kg soil dry weight and control was within a range of 25%
deviation to control; at 32 mg and 100 mg test item/kg soil dry weight
showed no negative effect on the nitrate-N content, but increased
nitrate-N contents compared to control, while 320 mg/kg and 1000 mg/kg
soil dw had a strong inhibiting effect on the nitrate-N concentrations.
Therefore, it can be concluded that the EC50 is between 100 mg and 320
mg test item/kg soil dry weight. The statistical analysis revealed that
the NOEC for an inhibition of the nitrogen transformation in soil is 100
mg test item/kg soil dw.
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