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EC number: 251-718-8
CAS number: 33885-52-8
A study was performed to assess the effect
of the test material on the growth of the green algae Pseudokirchneriella
strain: NIVA CHL 1.The method followed that described in
the OECD TG No 201. Following a preliminary range-finding test, the
algae were exposed to a Saturated Solution (SS) prepared at a loading
rate of 100 mg/L and 4 dilutions of this SS (three replicate flasks per
concentration) and a control (six replicate flasks per concentration)
for 72 hours, under constant illumination and shaking at a temperature
between 22 and 23°C. Preparation of test solutionsstarted
with a loading rate of 100 mg/L applying one day of magnetic stirring in
a closed vessel in order to reach the maximum solubility of the test
item in the test medium. The resulting aqueous mixture was left to
stabilize for one hour whereafter the clear and colourless Saturated
Solution was siphoned out and used as highest test solution. The lower
test concentrations were prepared by subsequent dilutions of the SS in
test medium.Samples were taken from all treatments at t = 0
, 24, 48 and 72 h and analysed with a validated GC-FID method.All
during the test period. After 72 hours of exposure, the three lowest
test concentration were below the LOQ, while the
highest two concentrations were 0.16 and 0.53% of initial, respectively.Therefore
the geometric mean measured concentrations were calculated and used for
expression of endpoints. Statistically significant inhibition of growth
rate was found at the three highest geometric mean measured
concentration, starting at 0.10 mg/L. However, the effects at 0.10 and
0.23 mg/L, respectively, were biologically not relevant (<10%).
Therefore the NOErC was set at 0.23 mg/L. The ErC10 and ErC50 based on
geometric mean measured concentrations were 0.37 and >0.66 mg/L,
EC50 growth rate is > 0.66 mg/l
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