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EC number: 251-718-8
CAS number: 33885-52-8
A study was performed to assess the acute
toxicity of the substance to fish (Cyprinus carpio). The study
was conducted in accordance with OECD TG 203 and GLP. In a semi-static
test set-up in air-tight closed vessels (fill volume: 6.2 L), seven fish
per concentration were exposed to 5 concentrations of the substance in
test water (10, 18, 32, 56 and 100% of a saturated solution prepared at
a loading rate of 100 mg/L) and a control. Throughout the test, test
solutions were refreshed in intervals of 24 hours. After 3.5 hours all
fish in the two highest treatment groups had died. Hypoactive swimming
was observed in the test group at 0.76 mg/L after 24 and 48 hours,
whereas no abnormalities were observed after 3.5, 72 and 96 hours. In
the test group at 1.3 mg/L, incidences of mortality were observed within
24 hours, as well as loss of equilibrium and/or immobility at all other
timepoints. Based on mortality the following effect value was
determined: 96 -h LC50 = 1.5 mg/L. All results are expressed in terms of
mean measured concentrations. Initial measured concentrations in fresh
solutions at test start and at t=72 hours were comparable and decreased
in used solutions to 52 -78 % of initial during the first refreshment
period of 24 hours, and to 49 -54% of initial during the last
refreshment period of 24 hours. Therefore, geometric mean measured
concentrations were calculated for the first and last time interval, and
these values were used to calculate the 0 -96 h (arithmetic) mean
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