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EC number: 251-718-8
CAS number: 33885-52-8
Several Ready test results are available which will be presented one by
one in the additional information section.
Closed Bottle test OECD TG 301D: Not ready biodegradable
Extended Closed Bottle test (OECD TG 301D, non-GLP: Not persistent
MITI test (OECD TG 301C: Not readily biodegradable but full primary
degradation into the substance respective acid and alcohol
Ready biodegradability test, Closed Bottle test in activated sludge
The ready biodegradability was determined in the Closed Bottle test
(OECD TG 301D) under GLP. Activated sludge is exposed to 2 mg/l of test
substance. At day 7, 14, 21 and 28 days the following degradation rates
were measured 0, 0. 2 and 3%, respectively. The substance should
therefore be classified as not readily biodegradable. The substance
caused a slight inhibition of the endogenous respiration. This test in
activated sludge was prolonged (non-GLP) and the test substance was
biodegraded by 29% at day 60.
Ready biodegradability test, Closed Bottle test in river water and
Introduction and method: The ready biodegradability of Pinyl
isobutyraldehyde was investigated in a series of Closed Bottle tests
according to OECD TG301D, not under GLP. The goal of this study was to
investigate the influence of the source of inoculum and test substance
concentration on the biodegradability of the substance. Tests were
performed with activated sludge and river water as inocula, and with
test substance concentrations of 1 and 2 mg/L.
Results: Inhibition of the endogenous respiration of the inoculum
during the first 2 weeks was detected in the presence of the substance
independent on the type of inoculum and concentration, except in river
water and 1 mg/l concentration no inhibition was seen. The following
results were seen at day 7,14, 21, 28, 42, 56 and 70.
Activated sludge 2 mg/l: -2, -2, 1, 5, 12, 32, 66%, respectively;
River water 2 mg/l: -3,-1, 6, 14, 32, 47 and 66%, respectively;
River water 1 mg/l: -1, 0, 2, 18, 54, 73 (at 56 days) and last
time point not measured, respectively (key value is underlined).
Discussion and Conclusion: Biodegradation percentages of < 20
were found at day 28 with both river water and activated sludge as
inocula. The substance is therefore not readily biodegradable. After 56
to 70 days biodegradation percentage in excess of 60 were found in a
prolonged Closed Bottle test. Higher biodegradation rates were found
with river water compared to the tests performed with activated sludge
as inoculum. A result of >60% was obtained with river water and an
initial test substance concentration of 1.0 mg/L and therefore the
substance is not persistent.
Ready biodegradability in MITI test:
In order to assess the biodegradation of the substance a modified MITI
test according to OECD 301C was performed and a "Method for Testing the
Biodegradability of Chemical Substances by Microorganisms" stipulated in
the "Testing Methods for New Chemical Substances", was applied under GLP
conditions. In this study a mixture of activated sludge (containing
samples from surface water and surface soil of rivers, lakes and inland
sea, and return sludge from sewage plants) was exposed to 100 mg/L of
the substance for 28 days. The validity criteria of the test were met.
Though it needs to be noted that in the concentration of the test item
in the test exceeded its water solubility (100 versus 10 mg/l,
respectively) and the pH was not measured to limit the volatilisation
from the test vessels. The test item was measured and ca 85% could be
recovered, the ca 15% loss was probably due to volatilisation. The test
item was not biodegraded (-1%) during the 28 -days and is therefore not
readily biodegradable. The substance slightly decreased the oxygen
consumption of the bacteria, which is an indication of the toxicity of
the substance or its metabolites. The test item was fully primary
degraded and the metabolites were identified: the aldehyde functional
group was converted to an acid and/or an alcohol. The concentration of
these two could not be quantified and not be distinguished. The pH was
not measured to limit the volatilisaton of the test item.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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