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EC number: 259-134-5
CAS number: 54381-16-7
of LD50 by the Weil Method (Biometrics, Sep. 1952)
level (mg/kg bw) Mortality
ratio = 2.0
= Lowest dose level
= log of constant
f = 0.2
values = 0, 1, 5, 5
M = log Da + d (f+1)
M =0 + 0.3010 (0.3+1)
M = 0 + 0.3010 (1.3)
M = 0.3913
of M = 246.2 (LD50)=246
of confidence limits = log M ± 2d (delta f)
= 0.3913± 2 (0.3010) (0.2)
0.5117 = 324.8
objective of this study was to determine the acute oral toxicity of
N,N-Bis(2-Hydroxyethyl)-p-Phenylenediamine Sulfate when administered
once orally to male rats.
total of 20 male rats weighing 250-300 g were divided into four
different treatment groups containing 5 rats each. The test substance
was prepared in dimethyl sulfoxide (DMSO; 10%) and administered orally
at dose levels of 100, 200, 400 and 800 mg/kg bw.
the study period animals were observed for mortality.
the animals in dose groups 400 and 800 mg/kg bw died during the study
period. During the study, one of 200 mg/kg bw died during the study
period. No mortality was observed at dose level of 100 mg/kg bw
throughout the observation period. Clinical signs were not reported in
of test substance was calculated by the Weil Method (Biometrics, Sep.
1952) and was determined to be 246 mg/kg bw with 95% confidence limit of
on above, the acute oral LD50 of
N,N-Bis(2-Hydroxyethyl)-p-Phenylenediamine Sulfate was determined to be
246 mg/kg bw (95% CL: 187-325) when administered at dose levels of 100,
200, 400 and 800 mg/kg bw in male rats.
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