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Environmental fate & pathways

Biodegradation in water and sediment: simulation tests

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Reference
Endpoint:
biodegradation in water: sewage treatment simulation testing
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
From Dec. 14, 2001 to July 1, 2002
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Followed guideline, sufficiently detailed report
Qualifier:
equivalent or similar to
Guideline:
EPA OPPTS 835.3220 (Porous Pot Test)
Deviations:
no
GLP compliance:
no
Radiolabelling:
no
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge: Activated sludge was collected from aeration basin of Fairfield Wastewater Treatment Plant on Feb. 13, 2002. This STP receives predominantly domestic waste. Waste water (used as porous pot influent) was collected at least twice per week from the same wastewater treatment facility.
- Preparation of inoculum for exposure: The total suspended solids level of sludge was adjusted to approx. 3,000 mg/L at the test initiation. Wastewater was filtered through several layers of cheesecloth and stored under refrigeration
- Storage condition: The wastewater was processed as mentioned above and then stored under refrigeration until use.
- Storage length: Not reported
- Pretreatment: None
- Concentration of sludge: Not reported
- Initial cell/biomass concentration: Not reported
Duration of test (contact time):
47 d
Initial conc.:
ca. 20 mg/L
Based on:
other: TOC
Parameter followed for biodegradation estimation:
TOC removal
Details on study design:
TEST CONDITIONS
- Volume of test solution/treatment: Not reported
- Composition of medium: Wastewater (details in “Test System”)
- Additional substrate: No
- Test temperature: Not specified in the study report. The porous pots were conducted under ambient laboratory conditions, approx. 20 C (source: author of study report).
- pH: Not reported
- Suspended solids concentration: Approx. 300 mg/L of TSS
- Continuous darkness: No
- Any indication of the test material adsorbing to the walls of the test apparatus: Not reported

TEST SYSTEM
- Culturing apparatus: Stainless steel porous pots consisting of a porous stainless steel membrane, a glass jacket, and metering pumps. The apparatus retains the sludge solids but allows liquid to flow through the system.
- Number of culture flasks/concentration: 1
- Hydraulic retention time (HRT): Approx. 6 h (controlled by the influent rate using low-volume pumps)
- Sludge retention time: Approx. 10 d (controlled by removing sludge solids from the bioreactor)
- Operation of porous pot: The pots were amended with 10% freshly collected activated sludge weekly. The sludge was stirred continuously at medium speed to thoroughly mix and maintain the solids in suspension. Wastewater (influent) and test material solution were dosed directly to the activated sludge. Effluent passed through the porous pot and discharged through a side port of the outer jacket positioned approx. one inch from the top of the porous pot. The porous pots were allowed to operate for 2 SRTs to attain steady state. Sampling of effluent samples was performed following this acclimation period
- Test performed in open system: Yes

SAMPLING
- Sampling frequency: The samples were collected daily from each porous pot over six weeks (excluding weekends)
- Sampling method: 50-100 mL effluent samples were collected from each porous pot
- Sample storage before analysis: The samples were acidified to approximately pH 2 using HCl and refrigerated until analysis

CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes, pot receiving wastewater only
- Abiotic sterile control: No
- Toxicity control: No

EVALUATION: To assess removal, the treated pot effluent samples were analyzed for soluble organic carbon and compared to that of the control pot effluent samples

STATISTICAL METHODS: Not reported
Key result
% Degr.:
0
Parameter:
TOC removal
Remarks on result:
other: Average removal through the 47 day test period
Transformation products:
not measured
Evaporation of parent compound:
not measured
Volatile metabolites:
not measured
Residues:
not measured
Details on results:
TEST CONDITIONS
- Aerobicity, moisture, temperature and other experimental conditions maintained throughout the study: No details in study report.

REMOVAL PERCENT
- The mean percent removal of test material over a six week testing period was -11 ±16.01%.

For details, refer to ‘table 1’ under ‘Any other information on results incl. tables ‘

Table 1. Percent removal of N,N(bis)-2-hydroxyethyl-p-phenylenediamine sulphate (Porous pot test) (Study # 44407)

Dates

TC

IC

TOC

TOC less blank

Removal %

3/21/2002

34.22

9.37

24.85

18.25

-34.20

3/22/2002

28.93

3.41

25.53

18.15

4.34

3/25/2002

31.05

2.94

28.11

20.36

1.54

3/26/2002

44.98

16.07

28.91

23.81

-15.15

3/27/2002

47.59

15.1

32.49

26.71

-29.17

3/28/2002

30.92

7.73

23.19

18.33

1.37

4/2/2002

32.64

5.13

27.51

21.6

-16.23

4/3/2002

34.13

9.08

25.05

19.28

-3.78

4/4/2002

37.48

10.14

27.34

18.57

0.06

4/5/2002

31.03

2.08

28.95

22.15

-32.86

4/8/2002

44.66

13.08

31.58

25.06

-50.30

4/9/2002

36.31

6.94

29.37

23.13

-35.47

4/10/2002

39.68

5.75

33.94

25.15

-47.31

4/11/2002

37.33

6.8

30.53

23.89

-39.90

4/12/2002

38.26

9.27

28.98

22.6

-26.78

4/15/2002

34.88

5.82

29.06

19.99

-12.09

4/16/2002

31.53

0.48

31.06

24.86

-21.19

4/17/2002

35.58

8.15

27.43

21.19

-3.31

4/18/2002

30.19

1.03

29.17

22.5

-9.65

4/19/2002

32.29

1.7

30.59

24.74

-20.61

4/23/2002

37.66

0.69

36.97

26.34

-26.28

4/24/2002

32.63

3.5

29.13

22.25

-6.65

4/25/2002

22.6

0.06

22.54

17.76

14.87

4/29/2002

19.58

1.72

17.86

13.34

36.04

4/30/2002

26.54

4.15

22.39

17.63

1.15

5/1/2002

23.67

2.92

20.76

16.05

10.02

5/2/2002

23.41

2.53

20.88

16.46

7.76

5/3/2002

22.04

0.21

21.83

15.87

10.12

5/6/2002

24.14

1.54

22.6

18.34

-3.87

 

 

 

 

 

Mean =

-11 ±16.01

N,N(bis)-2-hydroxyethyl-p-phenylenediamine sulphate = A050 = CAS # 54381-16-7.

Validity criteria fulfilled:
not applicable
Conclusions:
No removal of N,N(bis)-2-hydroxyethyl-p-phenylenediamine sulphate (A050) was observed over a six week testing period in a porous pot removal test.
Executive summary:

A porous pot removal test was conducted on N,N(bis)-2-hydroxyethyl-p-phenylenediamine sulphate (A050), following the EPA OPPTS 835.3220 (Porous Pot Test) guideline. The test material was tested at approx. 20 mg TOC/L. The inoculum was activated sludge collected from aeration basin of Fairfield Wastewater Treatment Plant which receives predominantly domestic sewage. The test treatment and inoculum blank were measured singly.

Stainless steel porous pots containing activated sludge were continuously fed with domestic sewage and the test material. The pots were operated with an SRT (solids retention time) of 10 d and an HRT (hydraulic retention time) of 6 h.

The porous pots were allowed to operate for 2 SRTs to attain steady state. Following this acclimation period, effluent samples were collected periodically, analyzed for soluble organic carbon (SOC) and compared to that of a control porous pot receiving no test substance.

No removal of N,N(bis)-2 -hydroxyethyl-p-phenylenediamine sulphate was observed over a six week testing period in the porous pot removal test.

Description of key information

No removal of N,N(bis)-2-hydroxyethyl-p-phenylenediamine sulphate was observed over a six week testing period in a porous pot removal test.

Key value for chemical safety assessment

Additional information

A porous pot removal testwas conducted on N,N(bis)-2-hydroxyethyl-p-phenylenediamine sulphate (A050), following theEPA OPPTS 835.3220 (Porous Pot Test) guideline. The test material was tested at approx. 20 mg TOC/L. The inoculum was activated sludge collected from Taylor Creek Wastewater Treatment Plant which receives predominantly domestic sewage. 

Stainless steel porous pots containing activated sludge were continuously fed with domestic sewage and the test material. The pots were operated with an SRT (solids retention time) of 10 d and an HRT (hydraulic retention time) of 6 h.

The porous pots were allowed to operate for 2 SRTs to attain steady state. Following this acclimation period, effluent samples were collected periodically, analyzed for soluble organic carbon (SOC) and compared to that of a control porous pot receiving no test substance.

No removal of N,N(bis)-2 -hydroxyethyl-p-phenylenediamine sulphate was observed over a six week testing period in the porous pot removal test.