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EC number: 202-616-7
CAS number: 97-89-2
An Open Epicutaneous Test (OET) was
performed on guinea pigs to assess the skin sensitization potential of
The Pretest was performed to establish
the primary irritating threshold concentration of test substance. For
this, a single application of 0.025 ml of each test concentration (e.g,
100, 30, 10 and 3%) is simultaneously performed on one of the areas
measuring 2 cm2 of the flank skin previously clipped and marked with a
circular stamp. Reactions are read 24 h after the application of the
test material. On the basis of preliminary study, the maximal
non-irritating concentration selected was 4%. Thus the sensitization
test was conducted at a dose of 4% of test chemical.
On day 1 during induction, 0.1 ml of
test chemical was applied at concentrations of 4% in vehicle to an area
measuring 8 cm2 on the clipped flank skin of the guinea pigs. The
applications are repeated daily for 3 weeks or done 5 times weekly
during 4 weeks, usually on the same skin sites. The application sites
were left uncovered and the reactions, if continuous daily applications
were performed, can be read 24 h after each application, or at the end
of each week.
To determine whether or not contact
sensitization was induced, all groups of guinea pigs previously treated
for 21 days, as well as 10 untreated, or only pretreated with the
vehicle, controls are tested on days 21 and 35 on the contralateral
flank with the test material. This test was performed by applying with a
pipette 0.025 ml of each concentration to skin areas measuring 2 cm2.
The reactions were read after 24, 48 and/or 72h.
It was observed that none of the
guinea pigs induced contact sensitization at challenge concentration of
4%.Thus, the test chemical was considered to be not sensitizing on skin
of guinea pigs at concentration of 4% in an Open Epicutaneous Test (OET).
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