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EC number: 202-616-7
CAS number: 97-89-2
The adsorption coefficient Koc in soil
and in sewage sludge of test chemical was determined by the Reverse
Phase High Performance Liquid Chromatographic method according to OECD
Guideline No. 121 for testing of Chemicals.The solutions of the test
substance and reference substances were prepared in appropriate
solvents. A test item solution was prepared by accurately measuring 4 μL
of test item and diluted with acetonitrile up to 10 ml. Thus, the test
solution concentration was 350 mg/l. The pH of test substance was 6.15.
Each of the reference substance and test substance were analysed by HPLC
at 210 nm. After equilibration of the HPLC system, Urea was injected
first, the reference substances were injected in duplicate, followed by
the test chemical solution in duplicate. Reference substances were
injected again after test sample, no change in retention time of
reference substances was observed. Retention time tR were measured,
averaged and the decimal logarithms of the capacity factors k were
calculated. The graph was plotted between log Koc versus log k(Annex -
2).The linear regression parameter of the relationship log Koc vs log k
were also calculated from the data obtained with calibration samples and
therewith, log Koc of the test substance was determined from its
measured capacity factor. The reference substances were chosen according
to estimated Koc range of the test substance and generalized calibration
graph was prepared. The reference substances were having Koc value
ranging from 1.25 to 4.09. The Log Koc value was found to be 2.2724 ±
0.0003at 25°C. This log Koc value indicates that the test substance has
a low sorption to soil and sediment and therefore have moderate
migration potential to ground water.
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