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EC number: 202-616-7
CAS number: 97-89-2
The ready biodegradability
ofCitronellyl Isobutyrate (CAS no. 97-89-2) was determined by the OECD
Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test),
EU Method C.4-D (Determination of the "Ready" Biodegradability -
Manometric Respirometry Test) and EPA OPPTS 835.3110 (Ready
Biodegradability), respectively for 34 days (Sustainability Support
Services has a letter of access, 2012). Fresh activated sludge from a
biological waste water treatment plant treating predominantly domestic
sewage was used. The sludge is collected in the morning, washed three
times in the mineral medium (by centrifuging at 1000 g for 10 minutes,
discarding the supernant and resuspending in mineral medium) and kept
aerobic until being used on the same day. Initial test substance
concentration used in the study was 30 mg/l. Mineral medium was prepared
by mixing 50 ml of solution A and 2000 ml deionised water, adding 5 ml
of each of the solutions B, C and D and making up to 5 litres with
deionised water. The pH is measured and if necessary adjusted to 7.4 +/-
0.2 with phosphoric acid or potassium hydroxide. The water used during
this study is deionised water containing less than 10 mg/l dissolved
organic carbon. Suspended solids concentration used in the study was
5.46 g/l diluted to 1.53 g/l dry matter. Sodium benzoate (100 mg/l)
(purity min. 99.0%) was used as a reference substance for the study.
Degradation of sodium benzoate exceeded 40% after 7 days and 65% after
14 days. Citronellyl Isobutyrate undergoes 75% biodegradation after 28
days in the test conditions. The 10 day window criterion is also
fulfillled (15% biodegradation on day 2 and 69% biodegradation on day
12). Citronellyl Isobutyrate did not inhibit the intrinsic respiration
of the inoculum at the test concentration and was therefore considered
to be non-toxic to the inoculum at the test concentration. Thus,
Citronellyl Isobutyrate should be regarded as readily biodegradable
according to the test.
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