2-Butenoic acid, 4-(diethoxyphosphinyl)-2-methyl-, ethyl ester

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP, non-guideline study, available as unpublished report, limitations in design and/or reporting but otherwise adequate for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1976

Report date:

1976

Materials and methods

Principles of method if other than guideline:

Six rats per sex per dose were exposed to the saturated vapour of the test substance. After an observation period of 7 days, animals were necropsied.

GLP compliance:

no

Test type:

other: Inhalation risk test

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

4-(diethoxyphosphinyl)-2-methyl-2-butenoic acid ethyl ester

EC Number:

601-818-6

Cas Number:

122009-11-4

Molecular formula:

C11 H21 O5 P

IUPAC Name:

4-(diethoxyphosphinyl)-2-methyl-2-butenoic acid ethyl ester

Details on test material:

- Name of test material (as cited in study report): C-5-Phosphonester

Test animals

Species:

rat

Strain:

not specified

Sex:

not specified

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 206.3 g for males, 165.7 g for females

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

air

Details on inhalation exposure:

- An air stream was conducted through a 5-cm layer of the test substance at 20°C to achieve an atmosphere that was saturated with the vapour of the test substance.
- Rate of air: 200 L/h

Analytical verification of test atmosphere concentrations:

not specified

Duration of exposure:

8 h

Concentrations:

Saturated vapour: 0.09 mg/L

No. of animals per sex per dose:

2 groups of 6 animals (3 males and 3 females each)

Control animals:

no

Details on study design:

- Duration of observation period following administration: 7 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 0.09 mg/L air

Based on:

test mat.

Exp. duration:

8 h

Mortality:

No mortality was observed in all exposed rats.

Clinical signs:

other: No clinical signs of toxicity findings were observed.

Body weight:

see Table 1 in "any other information on results incl. tables"

Gross pathology:

No pathological effects were observed.

Any other information on results incl. tables

Table 1: Body weight

Days after	Males		Females
treatment	First group	Second group	First group	Second group
0	192.0	187.3	168.7	162.7
7	234.0	240.7	175.3	169.0

Applicant's summary and conclusion

Conclusions:

Exposure to the saturated vapour pressure for 8 hours to the test substance does not result in mortality.

Executive summary:

Six rats per sex were exposed to the saturated vapour (0.09 mg/L air) of the test substance for 8 hours. After an observation period of 7 days, animals were necropsied. No clinical signs, pathological effects or mortality was observed in all exposed animals.