2-Butenoic acid, 4-(diethoxyphosphinyl)-2-methyl-, ethyl ester

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP, similar to guideline study, available as unpublished report, minor restrictions in design and/or reporting buth otherwise adequate for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Principles of method if other than guideline:

According to BASF-internal method: Five Wistar rats per sex per dose were exposed once by oral gavage to the test substance in 5% aquaous CMC solution. After an observation period of 14 days, animals were necropsied.

GLP compliance:

no

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4-(diethoxyphosphinyl)-2-methyl-2-butenoic acid ethyl ester

EC Number:

601-818-6

Cas Number:

122009-11-4

Molecular formula:

C11 H21 O5 P

IUPAC Name:

4-(diethoxyphosphinyl)-2-methyl-2-butenoic acid ethyl ester

Details on test material:

- Name of test material (as cited in study report): C-5-Ester-phosphonat Techn.

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Dr. K. Thomae GmbH, D-7950 Biberach, FRG (Germany)
- Weight at study initiation: Initial mean body weight was 180 g for the males and 179 g for the females
- Fasting period before study: 16 hours (water remained available ad libitum)
- Housing: five per cage in stainless steel wire mesh cages, Type DK-III (Becker&Co, Castrop-Rauxel, Freiburg, Germany)
- Diet: standard diet (Kliba-labordiaet 343), ad libitum
- Water: tap water, ad libitum
- Acclimation period: at least 1 week

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20-24
- Humidity (%): 30-70
- Air: fully air-conditioned rooms
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

CMC (carboxymethyl cellulose)

Remarks:

0.5% aqueous

Details on oral exposure:

VEHICLE
- Concentration in vehicle: the test substance was emulsified in 0.5% aqueous carboxymethylcellulose to a final concentration of 20% (w/v) and administered by gavage.
- Amount of vehicle (if gavage): 10 mL/kg bw

Doses:

2000 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: recording of signs and symptoms several times on the day of administration, at least once each workday. Check for moribund and dead animals twice each workday and once on holidays.
- Necropsy of survivors performed: yes; withdrawal of food about 16 hours before sacrifice with CO2, then necropsy at the end of the observation period, or necropsy of all animals that died as soon as possible. All animals were subjected to gross-pathological examination.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

test mat.

Mortality:

No mortality observed in all exposed animals.

Clinical signs:

other: Dyspnea, apathy, staggering, salivation, and poor general state were observed in both males and females. In females, abnormal position, atonia, and paresis were observed additionally. Symptoms were observed as early as 15 minutes after dosing. Animals wer

Gross pathology:

No pathological findings were observed.

Any other information on results incl. tables

Table 1: Body weight 

Time period	Mean body weight for males	Mean body weight for females
At test begin	180 g	179 g
After 7 days	243 g	215 g
After 10 days	259 g	221 g

Applicant's summary and conclusion

Interpretation of results:

practically nontoxic

Remarks:

Migrated information

Conclusions:

Under the conditions of this test, the LD50 was determined to be >2000 mg/kg bw.

Executive summary:

Five Wistar rats per sex were exposed to 2000 mg/kg bw of the test substance dissolved in 0.5% aqueous carboxymethyl cellulose solution, via oral gavage. After an observation period of 14 days the surviving animals were necropsied. Several clinical signs were observed such as dyspnea, apathy, staggering, salivation, abnormal position, atonia, paresis, and a poor general state. No pathological effects and no mortality were observed. The LD50 was therefore determined to be >2000 mg/kg bw.