2-Butenoic acid, 4-(diethoxyphosphinyl)-2-methyl-, ethyl ester

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German standard DIN 38 412, part L15.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

4-(diethoxyphosphinyl)-2-methyl-2-butenoic acid ethyl ester

EC Number:

601-818-6

Cas Number:

122009-11-4

Molecular formula:

C11 H21 O5 P

IUPAC Name:

4-(diethoxyphosphinyl)-2-methyl-2-butenoic acid ethyl ester

Details on test material:

- Name of test material: CS-Ester-Phosphonate

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

The test solution was prepared by adding the test item to the test media without any pre-treatment. Subsequently the fish were placed into the test aquaria.

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Golden orfe
- Source: Fish farm Paul Eggers, D-2354 Hohenwestedt, Germany
- Length at study initiation: 6.7 cm (range 6.0 - 7.2 cm)
- Weight at study initiation: 2.8 g (range 2.0 - 3.6 g)
- Corpulence factor of the test fish batch: 0.9
- Housing: oil-free aerated and charcoal filtered tap water, flow-through system. Water temperature: 19 - 20 °C.
- Duration of housing: about 1 month
- Mortality during the last 2 weeks of housing: 0.03 %.
- Medical treatment: Twice with 0.05 mg/L malachite green chloride, once with 10 mg/L tetracycline hydrochloride
- Feeding during test: withdrawel of food 1 day before and during exposure.

ACCLIMATION
- Acclimation period: 3 days
- Type and amount of food: Growing feed F/B 50, ad libitum.
- Feeding frequency: daily
- Health during acclimation (any mortality observed): no mortality observed

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

2.5 mmol/L

Test temperature:

20 °C

pH:

7.4 - 7.7

Dissolved oxygen:

7.9 - 8.9 mg/L

Nominal and measured concentrations:

Nominal test concentrations: 0 (control), 100, 215, 464 and 1000 mg/L (based on the results of a range finding study).

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquaria (30 x 22 x 24 cm)
- Fill volume: 10 L
- Aeration: Continuous aeration with oil-free air
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 2.8 g fish/L test water

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, Part II, 1982; prepared from fully demineralised tap water (conductivity: max 10 micro MHO) by adding: 294.0 mg/L CaCl2·2H2O, 123.3 mg/L MgSO4·7H2O, 63.0 mg/L NaHCO3, 5.5 mg/L KCl
- Acid capacity: 0.8 mmol/L
- Ratio Na/K ions: 10 : 1
- Ca/mg ratio: 4:1

OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 hours day-night regime

EFFECT PARAMETERS MEASURED:
- Mortality and symptoms of intoxication after 1, 4, 24, 48, 72 and 96 h.

Reference substance (positive control):

yes

Remarks:

Chloroacetamide (determined Nov. 15, 1988)

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

681 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: The LC50 value was calculated as the geometrical mean of the LC0 and LC100 nominal values.

Details on results:

No undissolved test substance was visible within the test aquaria.
Symptoms: Apathy, narcotic-like state, tumbling

Results with reference substance (positive control):

LC50 after 48 hours: about 32 mg/L

Reported statistics and error estimates:

Finney, D.J., probit Analysis, Cambr. Univ. Press, 3rd ed., 1971 (slightly modified).

Any other information on results incl. tables

Sublethal observations / clinical signs:

Cumulative mortality:

Nominal Conc. (mg/L)	No. of fish	Dead fish after
		1h	4h	24h	48h	72h	96h
0	10	0	0	0	0	0	0
100	10	0	0	0	0	0	0
215	10	0	0	0	0	0	0
464	10	0	0	0	0	0	0
1000	10	3	10	10	10	10	10