Registration Dossier
Registration Dossier
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EC number: 601-818-6 | CAS number: 122009-11-4
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- GLP compliance:
- no
Test material
- Reference substance name:
- 4-(diethoxyphosphinyl)-2-methyl-2-butenoic acid ethyl ester
- EC Number:
- 601-818-6
- Cas Number:
- 122009-11-4
- Molecular formula:
- C11 H21 O5 P
- IUPAC Name:
- 4-(diethoxyphosphinyl)-2-methyl-2-butenoic acid ethyl ester
- Details on test material:
- - Name of test materia: C5-Ester-Phosphonate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- Municipal activated sludge from a laboratory-scale STP unit fed with urban waste water. The initial biomass concentration was 1 g/L dry solids.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
Test conditions
- Nominal and measured concentrations:
- Nominal test concentrations: 0 (control), 1, 10, 100, 250, 500, and 1000 mg/L.
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorphenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- ca. 650 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- With an EC20 value of >100 mg/L, disturbances in sewage treatment plants are not to be expected.
- Results with reference substance (positive control):
- - EC20: ca. 1 mg/L
- EC50 ca. 9 mg/L
- EC80: ca. 65 mg/L
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
